{"created":"2023-05-15T10:00:45.903531+00:00","id":5159,"links":{},"metadata":{"_buckets":{"deposit":"16d7ae52-066e-4672-aff5-2099497d4945"},"_deposit":{"created_by":5,"id":"5159","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"5159"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00005159","sets":["76","76:64"]},"author_link":["16378","34317","16381","4839","27883"],"control_number":"5159","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-12-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"173","bibliographicPageStart":"161","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"Chromosome Research"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"RbAp46/48, histone chaperone, is a family of evolutionarily conserved WD40 repeat-containing proteins, which are involved in various chromatin-metabolizing processes, but their in vivo functional relevance is yet unclear. In order to examine the biological role of pRbAp48 in chicken DT40 cells, we generated a tetracycline-inducible system for conditional RbAp48-knockout cells. Depletion of RbAp48 led to delayed S phase progression associated with slow DNA synthesis and nascent nucleosome formation, followed by accumulation in G2/M phase, finally leading to cell death. Prior to cell death, these cells exhibited aberrant mitosis such as highly condensed and abnormal chromosome alignment on the metaphase plate, leading to chromosome missegregation. Depletion of RbAp48 also caused dissociation of heterochromatin protein 1 (HP1) from pericentromeric heterochromatin. Furthermore, depletion of RbAp48 from cells led to elevated levels of acetylation and slightly decreased levels of methylation, specifically at Lys-9 residue of histone H3. These results suggest that RbAp48 plays an important role in chromosome stability for proper organization of heterochromatin structure through the regulation of epigenetic mark.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citation:\nSatrimafitrah P, Barman HK, Ahmad A, Nishitoh H, Nakayama T, Fukagawa T, Takami Y. RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability. Chromosome Res. 2016 May;24(2):161-73. doi: 10.1007/s10577-015-9510-8. Epub 2015 Dec 15. PMID: 26667624.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s10577-015-9510-8","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Springer Science+Business Media Dordrecht 2015","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09673849","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Pasjan, Satrimafitra","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27883","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Barman, Hirak Kumar","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ahmad, Ahyar","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"西頭, 英起","creatorNameLang":"ja"},{"creatorName":"ニシトウ, ヒデキ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishitoh, Hideki","creatorNameLang":"en"}],"familyNames":[{"familyName":"西頭","familyNameLang":"ja"},{"familyName":"ニシトウ","familyNameLang":"ja-Kana"},{"familyName":"Nishitoh","familyNameLang":"en"}],"givenNames":[{"givenName":"英起","givenNameLang":"ja"},{"givenName":"ヒデキ","givenNameLang":"ja-Kana"},{"givenName":"Hideki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34317","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00332627","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00332627"}]},{"creatorNames":[{"creatorName":"Nakayama, Tatsuo","creatorNameLang":"en"},{"creatorName":"中山, 建男","creatorNameLang":"ja"},{"creatorName":"ナカヤマ, タツオ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Nakayama","familyNameLang":"en"},{"familyName":"中山","familyNameLang":"ja"},{"familyName":"ナカヤマ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Tatsuo","givenNameLang":"en"},{"givenName":"建男","givenNameLang":"ja"},{"givenName":"タツオ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukagawa, Tatsuo","creatorNameLang":"en"}],"familyNames":[{"familyName":"Fukagawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Tatsuo","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16381","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"髙見, 恭成","creatorNameLang":"ja"},{"creatorName":"Takami, Yasunari","creatorNameLang":"en"},{"creatorName":"高見, 恭成","creatorNameLang":"ja"},{"creatorName":"タカミ, ヤスナリ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"髙見","familyNameLang":"ja"},{"familyName":"Takami","familyNameLang":"en"},{"familyName":"高見","familyNameLang":"ja"},{"familyName":"タカミ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"恭成","givenNameLang":"ja"},{"givenName":"Yasunari","givenNameLang":"en"},{"givenName":"恭成","givenNameLang":"ja"},{"givenName":"ヤスナリ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"16378","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80236356","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80236356"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-12-19"}],"filename":"Pasjan2016.pdf","filesize":[{"value":"2 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"Manuscript","objectType":"fulltext","url":"https://miyazaki-u.repo.nii.ac.jp/record/5159/files/Pasjan2016.pdf"},"version_id":"7c6ecfed-0d2b-4ed0-87b3-8ee5402323e7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chromatin assembly","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Heterochromatin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Histone chaperone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Histone modification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"WD40 protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["76","64"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-06-21"},"publish_date":"2020-06-21","publish_status":"0","recid":"5159","relation_version_is_last":true,"title":["RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2025-01-17T04:08:02.464068+00:00"}