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RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability
http://hdl.handle.net/10458/6009
http://hdl.handle.net/10458/60092488a8c6-9413-42fc-b6bd-d104abdb1a7c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Manuscript (2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2020-06-21 | |||||||||
| タイトル | ||||||||||
| タイトル | RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| キーワード | Chromatin assembly | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| キーワード | Heterochromatin | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| キーワード | Histone chaperone | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| キーワード | Histone modification | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| キーワード | WD40 protein | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Pasjan, Satrimafitra
× Pasjan, Satrimafitra× Barman, Hirak Kumar
× Ahmad, Ahyar
× 西頭, 英起× 中山, 建男× Fukagawa, Tatsuo× 髙見, 恭成 |
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | RbAp46/48, histone chaperone, is a family of evolutionarily conserved WD40 repeat-containing proteins, which are involved in various chromatin-metabolizing processes, but their in vivo functional relevance is yet unclear. In order to examine the biological role of pRbAp48 in chicken DT40 cells, we generated a tetracycline-inducible system for conditional RbAp48-knockout cells. Depletion of RbAp48 led to delayed S phase progression associated with slow DNA synthesis and nascent nucleosome formation, followed by accumulation in G2/M phase, finally leading to cell death. Prior to cell death, these cells exhibited aberrant mitosis such as highly condensed and abnormal chromosome alignment on the metaphase plate, leading to chromosome missegregation. Depletion of RbAp48 also caused dissociation of heterochromatin protein 1 (HP1) from pericentromeric heterochromatin. Furthermore, depletion of RbAp48 from cells led to elevated levels of acetylation and slightly decreased levels of methylation, specifically at Lys-9 residue of histone H3. These results suggest that RbAp48 plays an important role in chromosome stability for proper organization of heterochromatin structure through the regulation of epigenetic mark. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Citation: Satrimafitrah P, Barman HK, Ahmad A, Nishitoh H, Nakayama T, Fukagawa T, Takami Y. RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability. Chromosome Res. 2016 May;24(2):161-73. doi: 10.1007/s10577-015-9510-8. Epub 2015 Dec 15. PMID: 26667624. |
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| 言語 | en | |||||||||
| 書誌情報 |
Chromosome Research 巻 24, 号 2, p. 161-173, 発行日 2015-12-15 |
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| 出版者 | ||||||||||
| 出版者 | Springer | |||||||||
| 言語 | en | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 09673849 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isVersionOf | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.1007/s10577-015-9510-8 | |||||||||
| 権利 | ||||||||||
| 言語 | en | |||||||||
| 権利情報 | © Springer Science+Business Media Dordrecht 2015 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | AM | |||||||||
