@article{oai:miyazaki-u.repo.nii.ac.jp:00004862,
 author = {太田, 一良 and Ohta, Kazuyoshi},
 journal = {岩谷直治記念財団研究報告書},
 month = {Aug},
 note = {A yeast-like fungus Aureobasidium pullulans var. melanigenum strain ATCC 20524 produces an extracellular acidophilic xylanase with an optimum pH of 2.0. The 34 amino acid prepro-signal peptide of the A. pullulans xylanase gene （xynI） product expressed in the methylotrophic yeast Pichia pastoris directed the efficient secretion of 178 mg of active xylanase per liter of the culture medium. The three-dimensional model and mutational analysis of the xynI gene product showed that Asp-73 and Glu-157 residues located at the upper and lower edges of the active site cleft, respectively, play a significant role in its low pH optimum. Another extracellular xylanase was purifled from the culture supernatant of the A. Pullulans. The xylanase gene （xynII） encoded a 261-amino acid signal peptide and a 335-amino acid mature protein. The xynII5'-noncoding region had two consensus binding sites （5'-GCCARG-3'） for the transcription factor PacC mediating pH regulation. Quantitative real-time PCR analysis revealed that the transcription levels at pH 6.0 and pH 8.0 were8-fold and 22-fold higher than that at pH 2.7, respectively.},
 pages = {39--41},
 title = {バイオマスからの燃料エタノール生産を目的とした遺伝子組換え酵母の開発},
 volume = {29},
 year = {2006},
 yomi = {オオタ, カズヨシ}
}