{"created":"2023-05-15T10:00:28.297097+00:00","id":4778,"links":{},"metadata":{"_buckets":{"deposit":"ee74917f-4d59-4120-ba26-b92295223197"},"_deposit":{"created_by":5,"id":"4778","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"4778"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00004778","sets":["73","73:27"]},"author_link":["5570","5867","5574","5861","25948","5862","5868","25944"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"230","bibliographicPageStart":"223","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"Solvent Extraction Research and Development, Japan"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The use of AOT reverse micellar systems （RVMS） for solubilization and back-extraction of proteins has been studied in relation to their percolation phenomena by using the injection method. Back-extraction of cytochrome c and hemoglobin into the aqueous phase was difficult, while catalase, C.C.lipase and BSA were more easily back-extracted depending on the pH. The significance of electrostatic interaction between the micelles and proteins is confirmed by comparing extraction results and their percolation processes. A sharp increase in electrical conductivity caused by the percolation phenomenon well demonstrates intermicellar interactions or stability of RVMS mediated with solubilized proteins. The value of β, defined as the variation of percolation threshold with the concentration of solubilized proteins, can be utilized to evaluate these interactions and to explain the results of back-extraction. The proteins which have positive or negative β values tend to have weak or strong interactions with micelles which results in a higher or lower degree of back-extraction, respectively. The β values are also affected by pH as well as by molecular weight, hydrophobicity and the types of proteins solubilized in the micelles.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Association of Solvent Extraction"},{"subitem_publisher":"日本溶媒抽出学会"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13417215","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"久保井, 亮一"},{"creatorName":"クボイ, リョウイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"5861","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"駒沢, 勲"},{"creatorName":"コマサワ, イサオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"5862","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Shiomori, Koichiro","creatorNameLang":"en"},{"creatorName":"塩盛, 弘一郎","creatorNameLang":"ja"},{"creatorName":"シオモリ, コウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Shiomori, Kouichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Shiomori","familyNameLang":"en"},{"familyName":"塩盛","familyNameLang":"ja"},{"familyName":"シオモリ","familyNameLang":"ja-Kana"},{"familyName":"Shiomori","familyNameLang":"en"}],"givenNames":[{"givenName":"Koichiro","givenNameLang":"en"},{"givenName":"弘一郎","givenNameLang":"ja"},{"givenName":"コウイチロウ","givenNameLang":"ja-Kana"},{"givenName":"Kouichiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5570","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80235506","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80235506"}]},{"creatorNames":[{"creatorName":"河野, 恵宣"},{"creatorName":"カワノ, ヨシノブ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawano, Yoshinobu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5574","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuboi, Ryoichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5867","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hong, Dong-pyo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"25944","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Komasawa, Isao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5868","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Lee, Sung-sik","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"25948","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"somr_1996.pdf","filesize":[{"value":"375.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://miyazaki-u.repo.nii.ac.jp/record/4778/files/somr_1996.pdf"},"version_id":"d81e9385-fd84-465d-9852-20a6de82857a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of Proteins Solubilized into AOT Reverse Micelles on their Back-extraction and Percolation Processes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of Proteins Solubilized into AOT Reverse Micelles on their Back-extraction and Percolation Processes","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["73","27"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-06-21"},"publish_date":"2020-06-21","publish_status":"0","recid":"4778","relation_version_is_last":true,"title":["Effect of Proteins Solubilized into AOT Reverse Micelles on their Back-extraction and Percolation Processes"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2024-07-04T06:31:42.068926+00:00"}