@article{oai:miyazaki-u.repo.nii.ac.jp:00004667,
 author = {保田, 昌秀 and Yasuda, Masahide and 竹尾, 圭祐 and Takeo, Keisuke and 宇都, 卓也 and Uto, Takuya and 湯井, 敏文 and 松本, 朋子 and Matsumoto, Tomoko and 石井, 康之 and Ishii, Yasuyuki and 太田, 一良 and Ohta, Kazuyoshi and 竹尾, 圭祐 and Takeo, Keisuke and Nagai, Hayato and Yui, Toshifumi},
 issue = {3},
 journal = {Journal of Sustainable Bioenergy Systems},
 month = {Sep},
 note = {Napiegrass （Pennisetum purpureum Schumach） was treated with a low-moisture anhydrous ammonia （LMAA） pretreatment by adding an equal weight of water and keeping it under atmospheric ammonia gas at room temperature for four weeks. After the removal of ammonia and washing with water, a simultaneous saccharification and fermentation （SSF） was conducted for the LMAA-pretreated napiergrass （1.33 g） in a buffer solution （8 mL） using a mixture of a cellulase （80 mg） and a xylanase （53 mg） as well as the cell suspension （0.16 mL） of Saccharomyces cerevisiae. Ethanol and xylose resulted in 91.2% and 62.9% yields, respectively. The SSF process was scaled up using LMAA-pretreated napiergrass （100.0 g） to give ethanol （77.2%） and xylose （52.8%）. After the removal of ethanol, the pentose fermentation of the SSF solution （40 mL）, which contained 1.00 g of xylose, using cell suspension of Escherichia coli KO11 （70 mL） gave 86.3% yield of ethanol. Total ethanol yield reached 68.9% based on xylan （21.4 wt%） and glucan （39.7 wt%） of the LMAA-pretreated napiergrass.},
 pages = {179--185},
 title = {Enhancement of Ethanol Production from Napiergrass (Pennisetum purpureum Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment},
 volume = {3},
 year = {2013},
 yomi = {ヤスダ, マサヒデ and タケオ, ケイスケ and ウト, タクヤ and ユイ, トシフミ and マツモト, トモコ and イシイ, ヤスユキ and オオタ, カズヨシ and タケオ, ケイスケ}
}