{"created":"2023-05-15T10:30:45.637867+00:00","links":{},"metadata":{"_buckets":{"deposit":"3d99dc1f-1738-477e-8f58-a1e0a15c8777"},"_deposit":{"id":"460.1","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"460.1"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000460.1","sets":["74:26"]},"author_link":["2643","2646","2805","2648","16920","2650"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"490","bibliographicPageStart":"485","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"Journal of nutritional science and vitaminology","bibliographic_titleLang":"en"},{"bibliographic_title":"J. nutr. sci. vitaminol","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Using [35S]PAPS as the sulfate donor, we have detected a sulfotransferase from bovine heart which catalyzes the sulfation of tyrosine-containing peptides. The enzyme displayed optimal activity at pH 5.75 and 35°C in a one-hour reaction. The addition of 10 mM Mn2+ or Co2+ to the reaction mixture increased the sulfotransferase activity by 3.4 and 3.5-fold, respectively. In contrast, the maximum increment stimulated by Mg2+ was only 1.75-fold at 15 mM concentration, and instead of exerting an enhancement effect, Ca2+ was found to be a potent inhibitor. The addition of 50 mM NaF to the reaction mixture resulted in an increase in sulfotransferase activity of 3.3-fold. The Km for 3'-phosphoadenosine 5'-phosphosulfate （PAPS） was determined to be 2μM at a constant 0.5 mM Boc-Glu-Asp-Tyr-Val. Among the 10 peptides tested as substrates, Boc-Glu-Asp-Tyr-Val and Boc-Asp-Asp-Tyr-Val provided the highest activities.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本ビタミン学会","subitem_publisher_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00703822","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03014800","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suiko, Masahito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2643","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00128357","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00128357"}]},{"creatorNames":[{"creatorName":"Fernando, P. H. Prasantha","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2646","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakakibara, Yoichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2805","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90295197","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90295197"}]},{"creatorNames":[{"creatorName":"Kudo, Hisao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2648","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamura, Toyohiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16920","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Liu, Ming-Cheh","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2650","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"JNSV43.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JNSV43.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/460.1/files/JNSV43.pdf"},"version_id":"d3569359-d47a-48c1-ab8a-086d09cdb884"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bovine heart, Sulfotransferase, Tyrosine sulfation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of Bovine Heart Sulfotransferase Catalyzing the Sulfation of Tyrosine-Containing Peptides","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of Bovine Heart Sulfotransferase Catalyzing the Sulfation of Tyrosine-Containing Peptides","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-02-24"},"publish_date":"2009-02-24","publish_status":"0","recid":"460.1","relation_version_is_last":true,"title":["Characterization of Bovine Heart Sulfotransferase Catalyzing the Sulfation of Tyrosine-Containing Peptides"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-07-21T09:31:44.238972+00:00"}