@article{oai:miyazaki-u.repo.nii.ac.jp:00000441,
 author = {八幡, 昌紀 and Yahata, Masaki and 柏原, 夕希子 and 黒木, 宏憲 and 國武, 久登 and Kunitake, Hisato and 小松, 春喜 and Komatsu, Haruki and 八幡, 昌紀 and Yahata, Masaki and Kashihara, Yukiko and Kurogi, Hironori and 小松, 春喜 and Komatsu, Haruki},
 issue = {1},
 journal = {園芸学研究, Horticultural research (Japan)},
 month = {Apr},
 note = {ニンポウキンカンの種子を用いた試験管内染色体倍加について検討した.有糸分裂阻害剤であるコルヒチンとオリザリンを様々な濃度や時間で種子に処理し,その後500mg・liter^<-1>麦芽抽出物を添加したMT培地で培養した.処理2か月後,発芽率を調査し,フローサイトメーターと根端の染色体観察により得られた実生の倍数性を解析した.四倍体実生率は有糸分裂阻害剤の種類,処理濃度および時間に依存しており,本処理条件内では,コルヒチンの方がオリザリンより染色体倍加の効果が高かった.0.05%,48時間でコルヒチン処理を行った時,約50%と最も高い頻度で四倍体を獲得することができた.これらの四倍体の初期成長は貧弱であったが,3年生のカラタチ台に接ぎ木すると二倍体と同様に旺盛な成長を示した.以上のように,胚培養を組合せた種子へのコルヒチン処理は,ニンポウキンカンの四倍体を効率的に誘導できることが明らかとなった.
\nIn vitro chromosome doubling of nucellar embryos was investigated in Meiwa kumquat （Fortunella crassifolia Swingle）. Nucellar embryos were aseptically treated with two antimitotic agents, colchicine and oryzalin at different concentrations and times. After these treatments, nucellar embryos were successively cultured on MT medium containing 500 mg/liter^<-1> malt extract. Ploidy levels of seedlings were evaluated by both flow cytometry and chromosome counting of root tips. Frequency of tetraploid production depended on the kind of antimitotic agents as well as on the concentration and time of treatment. Among the treatment conditions tested, colchicine gave better results than oryzalin on inducing chromosome doubling. Tetraploid plants were obtained from approximately 50% of the nucellar seedlings treated with colchicine at 0.05% for 48 hours. These tetraploids showed poor growth during the early stage after acclimatization, but grew as vigorously as the diploid plants when grafted onto 3-year-old seedlings of trifoliate orange. These results showed that colchicine treatment combined with embryo culture is particularly efficient to induce chromosome doubling of nucellar embryos in Meiwa kumquat.},
 pages = {11--16},
 title = {ニンポウキンカン種子へのコルヒチンおよびオリザリン処理が四倍体植物誘導に及ぼす影響},
 volume = {3},
 year = {2004},
 yomi = {ヤハタ, マサキ and カシハラ, ユキコ and クロギ, ヒロノリ and クニタケ, ヒサト and コマツ, ハルキ and ヤハタ, マサキ and コマツ, ハルキ}
}