{"created":"2023-05-15T09:59:49.458122+00:00","id":4048,"links":{},"metadata":{"_buckets":{"deposit":"7d72782f-b874-42a0-83c9-cd56fed9fb2d"},"_deposit":{"created_by":9,"id":"4048","owner":"9","owners":[9],"pid":{"revision_id":0,"type":"depid","value":"4048"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00004048","sets":["74","74:59"]},"author_link":["21375"],"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Sugarcane (Saccharum spp. L.) is one of the most important agricultural crops whose\nproducts are both for export and domestic consumption in most of the tropical and\nsubtropical countries of the world. Several recent studies have confirmed that biological\nnitrogen fixation by endophytic bacteria contributes significantly to the nitrogen nutrition\nof sugarcane. Endophytic diazotrophic bacteria, which have been isolated in association\nwith sugarcane plants, includes Acetobacter diazotrophicus and Herbaspirillum spp. In\nthis research work, the main objective was to study the mechanisms involved in the\nestablishment of nitrogen fixing system in sugarcane plant through the investigation of\nthe mode of infection and mechanism by which the endophytic diazotrophic bacteria\ncolonizes sugarcane after inoculation.\nHerbaspirillum spp. B501gfp1 (B501gfp1), an isolate from wild rice was used as source\nof inoculum to investigate the interaction between nitrogen-fixing endophytic bacterium\nand micropropagated sugarcane plants under aseptic and non-sterile conditions. In the\nfirst place an investigation was carried out to detect if the green fluorescence protein's\nexpression from B501gfp1 could be distinguished from the sugarcane tissue's\nbackground fluorescence. B501gfp1 bacterial cells were spot inoculated on to sugarcane\ntissues from 5 month-old sterile micro-propagated plants. Stem tissues and leaf sections\nmounted on glass slides were directly inoculated with a single touch using the tip of a\nsyringe previously dipped into the inoculum containing 10(8) bacterial cells ml(-1). It was\nobserved that GFP fluorescence could be easily distinguished in the stem than in the leaf\ntissues. However, the brightness level of the fluorescence varied with time as a result of\nfluctuations in the bacterial cell density. The presence of chloroplasts in the leaf tissues of\nsugarcane requires the use of bright GFP variants when monitoring bacteria-plant\ninteractions using gfp labeled bacteria.\nIn a study under sterile condition, two Japanese sugarcane plant cultivars (cvs) NiF8 and\nNi15 were inoculated using B501gfp1 in 2 inoculum doses of 10(8) and 10(2) bacterial cells\nml(-1) suspension. The results obtained showed that bacterial cells colonized both the root\nand stem tissues, and colonization was apparent in the intercellular spaces. Higher\nbacterial numbers detected in plant tissues corresponded to the higher inoculum\nconcentration treatment. Bacterial numbers also varied between the 2 cultivars with the\nhigher values determined in cv. Ni15. Colonization ability of B501gfp1 in the presence\nof indigenous endophytes in sugarcane under non sterile condition was also carried out\nusing the two inoculum concentrations (10(2) and 10(8) bacterial cells ml(-1)) and in cv NiF8\nonly. Internal tissue colonization was observed in plants inoculated with both the 10(2) and\n10(8) B501gfp1 bacterial cells ml(-1) inoculum concentrations. However, extensive\ncolonization and higher bacterial numbers were determined in the basal stem tissues of\nplants inoculated with the 10(8) bacterial cells ml(-1). Infection and establishment of the\nbacteria in the plants varied with respect to the inoculation technique. In the sterile plants\ninoculated with intact roots and basal stem, more bacteria were detected in the root\ntissues with the lateral root junctions as the main entry points. However, in some\ninstances internal tissue colonization was observed to have occurred through intact root\nsurfaces. On the other hand, plants which were inoculated with their stem bases bearing\ncut openings under non-sterile condition had more bacteria colonizing the stem tissues\nwith dense establishments in the vascular bundle tissues. It can therefore be concluded\nthat the mode of infection and subsequent establishment mechanism in the internal tissue\nis facilitated by the inoculation technique and availability of entry points. This study also\nprovides evidence for non-specificity of host plant among endophytes since\nHerbaspirillum spp. B501gfp1, a rice isolate could colonize sugarcane plant tissues.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_textarea_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_textarea_value":"鹿児島大学大学院連合農学研究科博士論文"},{"subitem_textarea_language":"ja"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Njoloma, Joyce Prisca","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"21375","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"DT_JoycePriscaNjoloma.pdf","filesize":[{"value":"8.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DT_JoycePriscaNjoloma.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/4048/files/DT_JoycePriscaNjoloma.pdf"},"version_id":"42786c34-8dd2-4976-bf45-1c38dc3a3847"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Establishment Mechanism of Nitrogen Fixing System by Endophytic Bacteria in the Japanese Sugarcane Plant","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Establishment Mechanism of Nitrogen Fixing System by Endophytic Bacteria in the Japanese Sugarcane Plant","subitem_title_language":"en"},{"subitem_title":"内生菌による窒素固定システムがサトウキビに定着する仕組みの解明","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"9","path":["74","59"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-11-26"},"publish_date":"2007-11-26","publish_status":"0","recid":"4048","relation_version_is_last":true,"title":["Establishment Mechanism of Nitrogen Fixing System by Endophytic Bacteria in the Japanese Sugarcane Plant"],"weko_creator_id":"9","weko_shared_id":-1},"updated":"2023-07-30T23:01:03.588785+00:00"}