{"created":"2023-05-15T09:59:49.201529+00:00","id":4042,"links":{},"metadata":{"_buckets":{"deposit":"60995ffe-4e95-4c09-acf6-3b35c13339a3"},"_deposit":{"created_by":9,"id":"4042","owner":"9","owners":[9],"pid":{"revision_id":0,"type":"depid","value":"4042"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00004042","sets":["74","74:59"]},"author_link":["21367","21368"],"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Yellowtail, Seriola quinqueradiata were immunized with two different\nLactococcus garvieae bacterins, formalin-killed KG - phenotype cells\n(capsulated phenotype) and formalin-killed KG+ phenotype cells\n(non-capsulated phenotype). Inoculation of either of these two vaccines\nconferred long-term protection to yellowtail against challenge with the\ncapsulated Lactococcus garvieae strain. High agglutinating titres against\nKG+ phenotype cells persisted for 135 days but no or only low agglutinating\ntitres against KG - phenotype cells were detected in fish given either\nbacterin. These results suggest that a capsule on KG- phenotype cells\naffects its immunogenicity, but the antigens conferring protection against\nlactococcal infection to fish may be located on the surface of KG+ phenotype\ncells and not in the cell capsule. The protection offered by formalin-killed\nKG+ phenotype cell vaccine was not strain specific. Capsulated L. garvieae\ncells were phagocytosed well and fimbriae-like appendages were seen in KG\n- phenotype cells after treatment with immune serum.\nThe cell surface components of strains of Lactococcus garvieae were\nexamined. Two capsular types of L. garvieae were found; one with a highly\ndeveloped capsule (KG9408) and one with a micro-capsule (MS93003)\ncarrying fimbriae-like components projecting from cell surface. One strain\n(NSS9310) had neither cell capsule nor fimbriae-like structures on its cell\nsurface. The strains with the highly developed capsule were more virulent to\nfish than either the micro-capsular or non-capsular. The KG9408, MS93003\nand NSS9310 strains could be clearly differentiated by their susceptibility to\nbacteriophages. Protection against L. garvieae infection was induced in\nyellowtail, Seriola quinqueradiata by immunization with formalin-killed L.\ngarvieae KG9408 and MS93003 cells. Although protection was also induced\nby immunization with NSS9310, the level of protection was significantly\nlower than that with KG9408 and MS93003 vaccines. Passive immunization\nwith yellowtail immune sera raised against KG9408 and MS93003 conferred\nstrong protection to yellowtail with rapid bacterial clearance after challenge\nwith L. garvieae. Immunoblotting analysis of protein antigens extracted\nfrom L. garvieae strains using rabbit anti-KG9408 and anti-MS93003 sera,\nand yellowtail anti-KG9408 and anti-MS93003 sera indicated that some\nbands seen in KG9408 and MS93003 strains were not detectable in\nNSS9310.\nAn attenuated Lactococcus garvieae strain lacking a virulence-associated\ncapsule on its cell surface was evaluated for its application as a live vaccine.\nThe attenuated strain (MS93003A) was obtained from the parent strain\n(MS93003V), which produced a well-developed capsule, by culturing on an\nagar medium supplemented with 2,3,5-triphenyltetrazolium chloride (TTC).\nWhen live cells of L. garvieae (MS93003A) or formalin-killed cells\n(MS93003A) were used as an injectable vaccine, protection against virulent\nL. garvieae (MS93003V) was conferred on Seriola quiqueradiata.\nFurthermore, at a relatively lower water temperature and using a lower dose\nof the cells, application of live cells of L. garvieae (MS93003A) conferred a\nstronger immunity to fish when compared with that conferred by\nformalin-killed cells (MS93003A).\nThe MS93003A cells did not recover their virulence even after in ViVO\npassages in fish. MS93003A live cells also conferred long-lasting protective\nimmunity to S. quinqueradiata against virulent L. garvieae infection.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"ブリを魚病細菌Lactococcus garvieae(レンサ球菌)の英膜保有株(MS93003;KG\n-型)および莢膜欠失株(MS93003;KG+型)のホルマリン不活化菌体で免疫した｡\n両ホルマリン不活化菌体共に,ブリに長期の感染防御を誘導した｡ブリの血清中には,\nKG+株に対しては高い凝集抗体価が認められたが,KG-株には弱い凝集抗体価か全\nく凝集を示さなかった｡この結果,KG-株の莢膜は,ブリに対して免疫原性は低く,\nしかも防御抗原ではないと考えられた｡さらに防御抗原は,莢膜ではなく,KG+株の\n細胞表面に存在していることが示唆された｡ホルマリン不活化KG+菌体で免疫したブ\nリの感染防御には,株特異性は認められなかった｡莢膜を有する菌株であるKG-株は,\nワクチンしたブリの血清と反応させることで,ブリの貧食細胞に十分に貧食されること\nが判明した｡また,KG-株において,線毛様構造物が観察された｡\nレンサ球菌株の細胞表面構造について研究した｡その結果,大きな莢膜を有する菌株\n(KG9408)と微細莢膜を有する菌株(MS93003)が認められた｡両細菌にも,線毛様構\n造物が観察された｡また,英膜および線毛様構造物ともにまったく観察されない菌株\n(NSS9310)も認められた｡大きな莢膜を有する菌株(KG9408)のブリに対する毒性は,\n微細莢膜を有する菌株(MS93003)又は微細莢膜および莢膜も有しない菌株(NSS9310)\nよりも強かった｡バクテリオファージに対する感受性は,これら3株で明らかな違いが\n認められた｡さらに,KG9408株およびMS93003株のホルマリン不活化菌体は,ブリに\n防御免疫を誘導した｡しかし,NSS9310株の不活化菌体の免疫による防御免疫は,KG9408\n株およびMS93003株の免疫よりも顕著に低かったOぞれぞれのホルマリン不活化菌体で\n免疫したブリの血清を分離し,健康なブリにその血清を移入した｡その結果,KG9408\n株およびMS93003株で免疫したブリより分離した血清を移入した個体で,強い感染防\n御が観察された｡これらブリの免疫血清を用いて,それぞれの菌体の蛋白質を,免疫染\n色を施し解析した｡その結果,KG9408株およびMS93003株のみに認められるが,NSS9310\n株には発現していない蛋白のバンドが検出された｡\n莢膜を有する毒性の強いMS93003V株を,TTC(2,3,5-トリフェニルテトラゾリウムク\nロライド)を含有した寒天培地で継代培養することにより,元株のMS93003V株から\nMS93003A株を分離した｡MS93003A株は,莢膜は欠失しているが,線毛を細胞表面に保\n有している菌株であった｡MS93003A株は,ブリに対して病原性がないが,そのホルマ\nリン不活化菌体のブリへの免疫は,感染防御を強く誘導した｡そのため,病原性に関わ\nる莢膜を欠失させた菌株であるMS93003A株を弱毒生ワクチンに応用した｡その結果,\nMS93003A株の生ワクチンは,ホルマリン不活化菌体より免疫細菌数が低くても,また,\n低温下(17℃)で免疫しても十分な感染防御をブリに誘導することが確認できた｡さ\nらに,生体内通過を行った後でも病原性の回復は認められなかった｡","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10006_textarea_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_textarea_value":"鹿児島大学大学院連合農学研究科博士論文"},{"subitem_textarea_language":"ja"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大山, 剛","creatorNameLang":"ja"},{"creatorName":"オオヤマ, ツヨシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ooyama, Tsuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"dt_ohyama.pdf","filesize":[{"value":"5.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DT_大山剛.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/4042/files/dt_ohyama.pdf"},"version_id":"347fb295-fd48-44fb-8b7d-5ca36012d661"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"魚病細菌Lactococcus garvieaeに対するブリの免疫防御反応と弱毒生ワクチンのブリへの応用に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"魚病細菌Lactococcus garvieaeに対するブリの免疫防御反応と弱毒生ワクチンのブリへの応用に関する研究","subitem_title_language":"ja"},{"subitem_title":"The protective immune response to the bacterial fish pathogen, Lactococcus garvieae and application of its attenuated live L. garvieae cells in yellowtail, Sariola quinqueradiata","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"9","path":["74","59"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-11-22"},"publish_date":"2007-11-22","publish_status":"0","recid":"4042","relation_version_is_last":true,"title":["魚病細菌Lactococcus garvieaeに対するブリの免疫防御反応と弱毒生ワクチンのブリへの応用に関する研究"],"weko_creator_id":"9","weko_shared_id":2},"updated":"2023-07-30T23:00:35.869089+00:00"}