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水棲細菌を指標としたMultiplex TaqMan PCR法による溺死の補助診断法
http://hdl.handle.net/10458/4706
http://hdl.handle.net/10458/4706e1342fac-d167-4da1-a6f8-2b203b36061c
| 名前 / ファイル | ライセンス | アクション |
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論文要旨 (208.1 kB)
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学位論文審査結果の要旨 (82.5 kB)
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博士学位論文 (2.4 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2013-12-25 | |||||
| タイトル | ||||||
| タイトル | A new molecular approach to help conclude drowning as a cause of death : Simultaneous detection of eight bacterioplankton species using real-time PCR assays with TaqMan probes | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | 水棲細菌を指標としたMultiplex TaqMan PCR法による溺死の補助診断法 | |||||
| 言語 | ja | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Drowning, Real-time PCR, TaqMan probe, Bacterioplankton, Diatom test | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
内山, 岳人
× 内山, 岳人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We developed a novel tool for concluding drowning as a cause of death. We designed nine primer pairs to detect representative freshwater or marine bacterioplankton (aquatic bacteria) and then used real-time PCR with TaqMan probes to rapidly and specifically detect them. We previously cultured the genus Aeromonas, which is a representative freshwater bacterial species, in blood samples from 94% of victims who drowned in freshwater and the genera Vibrio and/or Photobacterium that are representative marine bacteria in 88% of victims who drowned in seawater. Based on these results, we simultaneously detected eight species of bacterioplankton (Aeromonas hydrophila, A. salmonicida; Vibrio fischeri, V. harveyi, V. parahaemolyticus; Photobacterium damselae, P. leiognathi, P. phosphoreum) using three sets of triplex real- time PCR assays and TaqMan probes labelled with fluorophores (FAM, NED, Cy5). We assayed 266 specimens (109 blood, 157 tissues) from 43 victims, including 32 who had drowned in rivers, ditches, wells, sea or around estuaries. All lung samples of these 32 victims were TaqMan PCR-positive including the lung periphery into which water does not readily enter postmortem. On the other hand, findings in blood and/or closed organs (kidney or liver) were PCR-positive in 84% of the drowned victims (except for those who drowned in baths) although the conventional test detected diatoms in closed organs in only 44% of the victims. Thus, the results of the PCR assay reinforced those of diatom tests when only a few diatoms were detectable in organs due to the low density of diatoms in the water where they were found. Multiplex TaqMan PCR assays for bacterioplankton were rapid, less laborious and high-throughput as well as sensitive and specific. Therefore, these assays would be useful for routine forensic screening tests to estimate the amount and type of aspirated water. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| ja | ||||||
| 本論文に関連する研究論文 Uchiyama T, Kakizaki E, Kozawa S, Nishida S, Imamura N, Yukawa N. A new molecular approach to help conclude drowning as a cause of death: simultaneous detection of eight bacterioplankton species using real-time PCR assays with TaqMan probes. Forensic Sci Int. 2012 Oct 10;222(1-3):11-26. doi: 10.1016/j.forsciint.2012.04.029. Epub 2012 Jun 7. PMID: 22682932. |
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| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士(医学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17601 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 宮崎大学 | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2013年度 | |||||
| 言語 | ja | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2013-09-20 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 医博甲第414号 | |||||
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| 関連名称 | http://hdl.handle.net/10458/4641 | |||||
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| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| リンク |
A new molecular approach to help conclude drowning as a cause of death : Simultaneous detection of eight bacterioplankton species using real-time PCR assays with TaqMan probes
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