{"created":"2023-05-15T09:56:43.126353+00:00","id":375,"links":{},"metadata":{"_buckets":{"deposit":"f4a8910c-1dc6-4812-ae90-58f41b3f5400"},"_deposit":{"created_by":5,"id":"375","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"375"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000375","sets":["74","74:26"]},"author_link":["1867","2006","1808","2012","2007","2011","6484"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-07-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"736","bibliographicPageStart":"731","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"The journal of veterinary medical science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pituitary folliculo-stellate （FS） cells were able to modify the effect of activin-A on gonadotropes through the paracrine factor, follistatin. The present study was aimed to examine whether a hypothalamic peptide, pituitary adenylate cyclase activating polypeptide （PACAP）, could be a regulator of this paracrine interaction. Co-culture of FS cell-originated cell line TtT/GF cells with rat anterior pituitary cells showed faint inhibitory effect on the stimulatory action of activin-A on FSH secretion. When PACAP was added to the culture during the co-culture period, however, the presence of TtT/GF cells caused significant suppression of the effect of activin-A on FSH secretion. Conditioned-media （CM） from TtT/GF cells, obtained by incubation of TtT/GF cells in the presence or absence of PACAP, were next added to the cultures of anterior pituitary cells alone. CM from TtT/GF cells without PACAP treatment revealed slight, but not significant, suppressive effect on activin-induced increases in FSH secretion and the percentage of FSH cells. Meanwhile, CM from PACAP-treated TtT/GF cells attenuated both effects of activin-A. Furthermore, the inhibitory effect of the CM was neutralized when follistatin antibody was present in the culture. These results suggest that PACAP is able to regulate the paracrine action of FS cells on pituitary gonadotropes. Besides expressing direct actions on pituitary endocrine cells, PACAP may have roles as a regulator of cell-to-cell interactions within the pituitary gland.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"下垂体濾胞星状細胞はパラクリン因子フォリスタチンを介してアクチビンの性腺刺激ホルモン産生細胞への作用を調節している可能性を示してきた.本研究では, このパラクリン作用の上位制御因子としての視床下部ペプチドホルモン, PACAPの役割を検討した.濾胞星状細胞の株化細胞TtT/GF細胞を下垂体前葉の初代培養細胞と共培養すると, アクチビンのFSH分泌刺激作用が若干抑えられたが, この間PACAPを同時に添加しておくとこの抑制は顕著なものとなった.次に, TtT/GF細胞をPACAPの存在下あるいは非存在下で培養することにより得られた培養液を下垂体培養細胞に加えた.PACAP非処理のTrT/GF細胞から得られた培養液は, アクチビンのFSH分泌及びFSH細胞数を増加させる作用に対して弱い抑制を示したが, これは有意な効果ではなかった.ところが, PACAP処理のTtT/GF細胞から得られた培養液はアクチビンの2つの作用を完全に抑えた.また, この培養液が示した抑制効果は, 培養液にフォリスタチン抗体を添加しておくと中和され, アクチビン作用が復元した.以上の結果から, PACAPは濾胞星状細胞の性腺刺激ホルモン産生細胞に対するパラクリン作用を調節できることが示唆された.PACAPは下垂体の内分泌細胞に対する直接的な作用に加え, 下垂体細胞間の相互作用の制御因子としての役割を有していると推察された.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Veterinary Science","subitem_publisher_language":"en"},{"subitem_publisher":"日本獣医学会","subitem_publisher_language":"ja"}]},"item_10001_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://ci.nii.ac.jp/naid/110003920429/"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://ci.nii.ac.jp/naid/110003920429/"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本獣医学会","subitem_rights_language":"ja"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである","subitem_rights_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10796138","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09167250","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Katayama, Tetsuro","creatorNameLang":"en"},{"creatorName":"片山, 徹郎","creatorNameLang":"ja"},{"creatorName":"カタヤマ, テツロウ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"中嶋, 倫子","creatorNameLang":"ja"},{"creatorName":"ナカシマ, ミチコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"喜屋武, 向子","creatorNameLang":"ja"},{"creatorName":"キヤン, ヒサコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村上, 昇","creatorNameLang":"ja"},{"creatorName":"ムラカミ, ノボル","creatorNameLang":"ja-Kana"},{"creatorName":"Murakami, Noboru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"黒田, 治門","creatorNameLang":"ja"},{"creatorName":"クロダ, ハルト","creatorNameLang":"ja-Kana"},{"creatorName":"Kuroda, Haruto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Katayama, Tetsuro","creatorNameLang":"en"},{"creatorName":"片山, 徹郎","creatorNameLang":"ja"},{"creatorName":"カタヤマ, テツロウ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Nakashima, Michiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kyan, Hisako","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"jvms62-7-731.pdf","filesize":[{"value":"918.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JVMS62(7)731.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/375/files/jvms62-7-731.pdf"},"version_id":"2c28941a-dd57-428a-b565-cd02b0aeb9a2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"activin, conditioned-media, gonadotrope, PACAP, TtT GF cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Role of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) as Regulator of Paracrine Interactions between Folliculo-Stellate Cells and Gonadotropes through the Control of Activin-Follistatin Interactions","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Role of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) as Regulator of Paracrine Interactions between Folliculo-Stellate Cells and Gonadotropes through the Control of Activin-Follistatin Interactions","subitem_title_language":"en"},{"subitem_title":"下垂体濾胞星状細胞と性腺刺激ホルモン産生細胞のパラクリン相互作用の調節因子としてのpituitary adenylate cyclase activating polypetide(PACAP)の役割:アクチビン-フォリスタチン制御系の調節を介して","subitem_title_language":"ja"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-05-27"},"publish_date":"2008-05-27","publish_status":"0","recid":"375","relation_version_is_last":true,"title":["A Role of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) as Regulator of Paracrine Interactions between Folliculo-Stellate Cells and Gonadotropes through the Control of Activin-Follistatin Interactions"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-10-31T04:34:05.821590+00:00"}