{"created":"2023-05-15T12:42:01.894843+00:00","links":{},"metadata":{"_buckets":{"deposit":"6b65b803-f2d2-4ff9-9433-b02feb596e05"},"_deposit":{"id":"373.1","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"373.1"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000373.1","sets":["74:26"]},"author_link":["932","1992","1993","1994"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"83","bibliographicPageStart":"77","bibliographicVolumeNumber":"121","bibliographic_titles":[{"bibliographic_title":"Comparative biochemistry and physiology. Part A, Molecular & integrative physiology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigated the effects of various protease substrates on the motility of demembranated fowl spermatozoa. In the presence of ATP, the motility of demembranated spermatozoa was vigorous at 30°C, but decreased markedly following the addition of protease substrates, such as Nα-carbobenzoxy-l-lys-thiobenzyl ester （BLT）, N-benzoyl-phe-val-arg p-nitroanilide or Nα-benzoyl-d,l-arg p-nitroanilide （BAPNA） in a dose-dependent manner, within the range 0-1 mM. The subsequent addition of 100 ng/ml trypsin released the inhibitory effect of protease substrates within 10 sec. Phosphorylation or dephosphorylation of several proteins of demembranated spermatozoa was observed following the addition of protease substrates, however, no consistent patterns of protein phosphorylation or dephosphorylation were associated with the inhibition of motility. These results suggest that endogenous protease activity is instrumental in the maintenance of fowl sperm motility and that the site of action of this protease is in the axoneme and/or accessory cytoskeletal components. This enzyme may not act directly on the phosphorylation of sperm proteins involved in the regulation of motility.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11183804","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10956433","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"芦沢, 幸二","creatorNameLang":"ja"},{"creatorName":"Ashizawa, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"932","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hashimoto, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1992","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Yoshie","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1993","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuzuki, Ysuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1994","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ashizawa64.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ashizawa64.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/373.1/files/ashizawa64.pdf"},"version_id":"c7cf2b30-dafa-4c00-b22c-c309b6e35712"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm, dephosphorylation, fowl, fragellar motility, phosphorylation, protease, temperature-dependent, spermatozoa, substrate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Inhibition of Flagellar Motility of Demembranated Fowl Spermatozoa by Protease Substrates","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibition of Flagellar Motility of Demembranated Fowl Spermatozoa by Protease Substrates","subitem_title_language":"en"},{"subitem_title":"Protease and fowl sperm motility","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-10-10"},"publish_date":"2007-10-10","publish_status":"0","recid":"373.1","relation_version_is_last":true,"title":["Inhibition of Flagellar Motility of Demembranated Fowl Spermatozoa by Protease Substrates"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-07-21T09:28:07.996347+00:00"}