{"created":"2023-05-15T09:59:33.701289+00:00","id":3712,"links":{},"metadata":{"_buckets":{"deposit":"076c8ed5-4436-4ace-b565-0ec174489c9d"},"_deposit":{"created_by":5,"id":"3712","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"3712"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00003712","sets":["73","73:51"]},"author_link":["5570","5867","5861","5862","5868"],"item_10003_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"116","bibliographicPageStart":"111","bibliographic_titles":[{"bibliographic_title":"Proceedings of Symposium on Solvent Extraction","bibliographic_titleLang":"en"}]}]},"item_10003_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Forward and back extraction of E.coli β-galactosidase(β - gal) were carried out using AOT-isooctane reverse micellar systems, in which an injection method and a phase transfer method were employed. β-Gal is a large oligomeric protein with molecular weight of 540kDa. Unexpectedly, β-gal was easily solubilized with the former method and back extracted into 0.1M KCI aqueous solution without much loss of enzyme activity, though β-gal in the micellar solution did not show enzyme activity. In the latter method, β-gal was extracted into the reverse micellar solution up to 40% of the feed protein depending on both salt type and salt concentration. A novel solubilization mechanism was suggested for the large oligomeric proteins.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10003_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本溶媒抽出学会","subitem_publisher_language":"ja"},{"subitem_publisher":"Japanese Association of Solvent Extraction","subitem_publisher_language":"en"}]},"item_10003_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Shiomori, Koichiro","creatorNameLang":"en"},{"creatorName":"塩盛, 弘一郎","creatorNameLang":"ja"},{"creatorName":"シオモリ, コウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Shiomori, Kouichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Shiomori","familyNameLang":"en"},{"familyName":"塩盛","familyNameLang":"ja"},{"familyName":"シオモリ","familyNameLang":"ja-Kana"},{"familyName":"Shiomori","familyNameLang":"en"}],"givenNames":[{"givenName":"Koichiro","givenNameLang":"en"},{"givenName":"弘一郎","givenNameLang":"ja"},{"givenName":"コウイチロウ","givenNameLang":"ja-Kana"},{"givenName":"Kouichiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5570","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80235506","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80235506"}]},{"creatorNames":[{"creatorName":"久保井, 亮一","creatorNameLang":"ja"},{"creatorName":"クボイ, リョウイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"5861","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"駒沢, 勲","creatorNameLang":"ja"},{"creatorName":"コマサワ, イサオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"5862","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuboi, Ryoichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5867","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Komasawa, Isao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5868","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"shiomori_1992.pdf","filesize":[{"value":"790.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"shiomori_1992.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/3712/files/shiomori_1992.pdf"},"version_id":"00715f45-207b-45da-8194-9da6ebd2f2b3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"solvent extraction","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"Reverse Micellar Extraction of β-Galactosidase from E.coli","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Reverse Micellar Extraction of β-Galactosidase from E.coli","subitem_title_language":"en"}]},"item_type_id":"10003","owner":"5","path":["73","51"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-11-01"},"publish_date":"2013-11-01","publish_status":"0","recid":"3712","relation_version_is_last":true,"title":["Reverse Micellar Extraction of β-Galactosidase from E.coli"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2024-07-04T06:31:33.821271+00:00"}