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Reverse Micellar Extraction of β-Galactosidase from E.coli
http://hdl.handle.net/10458/4639
http://hdl.handle.net/10458/4639f23aaa03-241c-4d04-b41c-8fa2bc5ce61d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
shiomori_1992.pdf (790.2 kB)
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| Item type | 会議発表論文 / Conference Paper(1) | |||||||||||||
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| 公開日 | 2013-11-01 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Reverse Micellar Extraction of β-Galactosidase from E.coli | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | solvent extraction | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||||||||||
| 資源タイプ | conference paper | |||||||||||||
| 著者 |
塩盛, 弘一郎
× 塩盛, 弘一郎
WEKO
5570
× 久保井, 亮一× 駒沢, 勲× Kuboi, Ryoichi× Komasawa, Isao |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Forward and back extraction of E.coli β-galactosidase(β - gal) were carried out using AOT-isooctane reverse micellar systems, in which an injection method and a phase transfer method were employed. β-Gal is a large oligomeric protein with molecular weight of 540kDa. Unexpectedly, β-gal was easily solubilized with the former method and back extracted into 0.1M KCI aqueous solution without much loss of enzyme activity, though β-gal in the micellar solution did not show enzyme activity. In the latter method, β-gal was extracted into the reverse micellar solution up to 40% of the feed protein depending on both salt type and salt concentration. A novel solubilization mechanism was suggested for the large oligomeric proteins. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : Proceedings of Symposium on Solvent Extraction p. 111-116, 発行日 1992-12 |
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| 出版者 | ||||||||||||||
| 出版者 | 日本溶媒抽出学会 | |||||||||||||
| 言語 | ja | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | Japanese Association of Solvent Extraction | |||||||||||||
| 言語 | en | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
