@article{oai:miyazaki-u.repo.nii.ac.jp:00003274,
 author = {村上, 隆之 and Murakami, Takayuki and 斎藤, 勇夫 and Saito, Isao and 芦沢, 広三 and Ashizawa, Hirozo},
 issue = {2},
 journal = {宮崎大学農学部研究報告, Bulletin of the Faculty of Agriculture, Miyazaki University},
 month = {Dec},
 note = {馬･牛･山羊･羊･犬･猫および狸の肝類洞壁の微細構造を観察し次の所見を得た.
 1. これら全ての動物において,類洞と肝細胞との間には単層に配列する血管内皮細胞とDisse腔が区分され,Disse腔内には肝細胞のmicrovilli,細網線維束,ある種の細胞片が認められる.
 2. 類洞壁の大部分を構成する薄板状の内皮細胞層には,大小種々の間隙および隔膜を欠除する有窓部が認められる.しかし,隣接する内皮細胞が互いに接触または重積し,単純隣接(接触)や中間結合(接着帯)などの弱い細胞間結合を示す像がしばしば認められる.また馬･羊･猫では,内皮細胞の有窓部に隔膜を保有するものも認められる.これらの所見から,肝類洞壁を構成する内皮細胞は本来,隔膜のある有窓内皮細胞の連続性であるが,切片作製過程における何らかの影響で細胞間結合が分離し,また有窓部の隔膜も破壊される結果,大小種々の間隙や隔膜のない有窓部を持つ内皮細胞層として認められるのであろう.
 3. 牛･山羊･羊では類洞内皮細胞層とDisse腔との間には連続的な基底膜が存在する., The fine structure of the hepatic sinusoidal walls were examined with an electron microscpe. Liver tissue obtained from two adult horses, two adult cows, three adult goats, two adult sheep, three adult dogs, three cats (two months to two years old) and two adult racoon dogs were cut into small pieces and fixed in conventional ice-cold 2%OsO4 buffered with s-Collidine at pH 7.4.  After 2 to 5 hours of fixation, the tissue blocks were dehydrated through etanol and aceton series, and embedded in Epon 812. Ultra-thin sections were double-stained in 2% uranyle acetate and Raynold's lead citrate, and examined in a Hitachi HU-11 D-S electron microscope. All thick sections of Epon embedded material stained with Yamamoto's toluidine blue did not show any significant pathological change. The electron microscopic observations are summarized as follows:
    1. In all the examined mammals, the Disse's space and vascular endothelial lining are distinguishable between the lumen of sinusoid and liver cells, and abundant microvilli protruded from the hepatocytes, reticular fibers and bits of unidentifiable cytoplasm are commonly contained in the space of Disse.
    2. In all of the livers numerous gaps and fenestrae, having no diaphragm, of variable width exist in the attenuated endothelial plates forming the largest portion of the sinusoidal walls, and sinusoidal linings are seen to be discontinuous (Figs. 2, 3 and 4). This is the hepatic sinusoid described in many electron microscopic studies. However, the free edges of the adjacent endothelial plates are apposed or overlapped, and between their cell membranes the structures of loose cell junction such as "simple apposition or contact" (Fig. 5) or "intermediate junction or zonula adhaerens" (Fig. 6) are frequently recognized. In some areas of liver from horses, sheep and cats, moreover, the diaphragms closing the fenestrae of sinusoidal linings are seen (Figs. 5 and 6).
    These findings suggest that the liver sinusoidal lining is continuous itself, but in the electron microscope the discontinuities are observed in the lining by detachment of the cell connection between the endothelial cells and destruction of the diaphragms closing the fenestrae during the tissue preparation.
    3. In the liver of cows (Fig. 2), goats (Figs. 1 and 4) and sheep (Fig. 5), the sinusoidal endothelium possesses a continuous basement membrane.},
 pages = {389--396},
 title = {肝類洞壁の微細構造について},
 volume = {19},
 year = {1972}
}