@article{oai:miyazaki-u.repo.nii.ac.jp:00003256,
 author = {八幡, 昌紀 and Yahata, Masaki and 関本, 貴則 and 安田, 喜一 and Yasuda, Kiichi and 小松, 春喜 and Komatsu, Haruki and 國武, 久登 and Kunitake, Hisato and 八幡, 昌紀 and Yahata, Masaki and Sekimoto, Takanori and 安田, 喜一 and Yasuda, Kiichi and 小松, 春喜 and Komatsu, Haruki},
 journal = {宮崎大学農学部研究報告, Bulletin of the Faculty of Agriculture University of Miyazaki},
 month = {Feb},
 note = {The possibility of using somatic hybrid 'Citrus parental line No.4' (Yuzu, Citrus junos Siebold. ex Tanaka+ navel orange, C sinensis (L.) Osbeck var. brasiliensis Tanaka) for triploid production was investigated by crossing it with diploid 'Banpeiyu' pummelo. 'Citrus parental line No.4' showed high pollen fertility as assessed by stainability (88.2 %) and germination rate (21.2 %), although one of the parents of this somatic hybrid was a male sterile navel orange. When the 'Banpeiyu' pummelo (C. maxima (Burm.) Merr.) was pollinated with pollen of 'Citrus parental line No.4', triploid seedlings were predominantly obtained from developed seeds at a high frequency (83.3 %), i.e., 105 triploid, 20 tetraploid, and 1 hexaploid seedlings. No clear correlation was observed between the weight of the seeds and the ploidy level of the seedlings, and triploids, tetraploids, and hexaploid were obtained from seeds with weights of 0.2-0.8 g, 0.4-0.6 g, and 0.2 g, respectively. In contrast, all of the seedlings obtained from undeveloped seeds were triploids. These results indicate that 'Citrus parental line No.4' can be useful as a parent for triploid breeding., ユズ (Citrus junos Siebold ex Tanaka) とネーブルオレンジ(C. sinensis(L.) Osbeck var. brasiliensis Tanaka) との体細胞雑種である`カンキツ中間母本農4号'を三倍体育種に利用するために,`晩白柚'と交雑を行い, 得られた実生の倍数性を調査した.`カンキツ中間母本農4号'の花粉稔性は, 片親のネーブルオレンジに花粉が全くないにも関わらず, 染色稔性と発芽稔性がそれぞれ88.2と21.2%であり, 高い稔性を示した.  そこで,`カンキツ中間母本農4号'を`晩白柚'に交雑した結果, 完全種子からは三倍体(105個体), 四倍体(20個体)および六倍体(1個体)の実生が得られ, 三倍体(83.3%)が多く出現した.  種子の重さと倍数性との間に相関は認められなかったが, やや大きい種子(0.4～0.6g)から四倍体が, 小さい種子(0.2g)から六倍体が出現していたのに対し, 三倍体は種子の重さに関係なくいずれの重さの種子(0.2～0.8g)からも発生していた.  なお,不完全種子から得られた実生の倍数性は全て三倍体であった.  本研究の結果より,`カンキツ中間母本農4号'は三倍体育種の親として利用できることが示唆された.},
 pages = {63--70},
 title = {Relationship between weight of seeds and the ploidy level of the seedlings obtained from the cross between 'Banpeiyu' pummelo and somatic hybrid 'Citrus parental line No.4'},
 volume = {57},
 year = {2011},
 yomi = {ヤハタ, マサキ and セキモト, タカノリ and ヤスダ, キイチ and コマツ, ハルキ and クニタケ, ヒサト and ヤハタ, マサキ and ヤスダ, キイチ and コマツ, ハルキ}
}