{"created":"2023-05-15T09:59:08.677311+00:00","id":3227,"links":{},"metadata":{"_buckets":{"deposit":"a54845c4-5bd7-4510-8659-8ec05d754583"},"_deposit":{"created_by":5,"id":"3227","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"3227"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00003227","sets":["74","74:46","74:46:349","74:46:349:362"]},"author_link":["16934","24982","16931","2632","16920","16929"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"58","bibliographicPageStart":"49","bibliographicVolumeNumber":"48","bibliographic_titles":[{"bibliographic_title":"宮崎大学農学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Faculty of Agriculture, Miyazaki University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Exo- and endoinulinases were extracted from mycelia of Aspergillus niger strain 12 using quartz sand and purified by DEAE-Cellulofine A-500, Sephadex G-1OO, and Sephadex G-200 \nchromatographies. The intracellular enzymes were homogeneous as judged by SDS-polyacrylamide gel electrophoresis. The purified exoinulinase P-II had specific activities of 6.6 U/mg toward inulin and 22 U/mg toward sucrose. The endoinulinase P-III showed 108 U/mg toward inulin, but no activity toward sucrose. Mrs of exoinulinase P-II and endoinulinase P-III were determined by gel filtration using Sephadex G-200 as 47 and 56 kDa, respectively. Optimal pH and temperature for enzyme activity were pH-5.0 and 55 ℃ for P-II, and pH 5.3 and 45℃ for P-III. Both the enzymes were activated by Mn2+ , and inactivated by Ag+, Hg2+, or p-chloromercuribenzoate. Inulinases P-II and P-III exhibited apparent Km values of 5.8 and \n0.80 mM, respectively.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"黒麹菌Aspergillus nigerNo.12株の培養菌体を石英砂で破砕し,その抽出液から細胞内エキソおよびエンド型イヌリナーゼをDEAE-セルロファインA-500,続いてセファデックスG-100およびG-200クロマトグラフィーによりそれぞれ電気泳動的に単一に精製した。エキソ型イヌリナーゼP-IIの比活性は,イヌリンに対して6.6U/mg,ショ糖に対して22U/mgであった。エンド型イヌリナーゼP-IIIはイヌリンのみに作用し,比活性は108U/mgであった。\nゲルろ過で測定した分子量は,エキゾ型イヌリナーゼP-IIが47kDa,エンド型イヌリナーゼP-IIIが56kDaであった。エキソ型イヌリナーゼP-IIとエンド型イヌリナーゼP-IIIの最適pHはそれぞれpH5.0とpH5.3,最適温度はそれぞれ55℃と45℃であった。両酵素ともMn2+で賦活化され,Ag+,Hg2+,p-クロロメルクリ安息香酸で失活した。エキソ型イヌリナーゼP-IIとエンド型イヌリナーゼP-IIIのイヌリンに対するMichaelis定数(Km)は,それぞれ5.8mMと0.80mMであった。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宮崎大学農学部","subitem_publisher_language":"ja"},{"subitem_publisher":"Miyazaki University","subitem_publisher_language":"en"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00236503","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05446066","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中村, 豊彦","creatorNameLang":"ja"},{"creatorName":"ナカムラ, トヨヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakamura, Toyohiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"倉盛, 宏一","creatorNameLang":"ja"},{"creatorName":"クロモリ, コウイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"在田, 典弘","creatorNameLang":"ja"},{"creatorName":"ザイタ, ノリヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Zaita, Norihiro","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"秋元, 秀俊","creatorNameLang":"ja"},{"creatorName":"アキモト, ヒデトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Akimoto, Hidetoshi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"太田, 一良","creatorNameLang":"ja"},{"creatorName":"オオタ, カズヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"Ohta, Kazuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuramori, Koichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"在田, 典弘","creatorNameLang":"ja"},{"creatorName":"ザイタ, ノリヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Zaita, Norihiro","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"秋元, 秀俊","creatorNameLang":"ja"},{"creatorName":"アキモト, ヒデトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Akimoto, Hidetoshi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"KJ00000503394.pdf","filesize":[{"value":"461.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000503394.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/3227/files/KJ00000503394.pdf"},"version_id":"c80b3766-a735-4467-80ca-268d5546415f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Aspergillus niger, Intracellular enzyme, Inulin, Inulinase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞内酵素, イヌリン, イヌリナーゼ","subitem_subject_language":"ja"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and Properties of Intracellular Exo- and Endoinlulinases from Aspergillus niger Strain 12","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and Properties of Intracellular Exo- and Endoinlulinases from Aspergillus niger Strain 12","subitem_title_language":"en"},{"subitem_title":"Aspergillus niger No.12株の細胞内エキソおよびエンド型イヌリナーゼの精製と性質","subitem_title_language":"ja"}]},"item_type_id":"10002","owner":"5","path":["74","46","349","362"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-02-03"},"publish_date":"2009-02-03","publish_status":"0","recid":"3227","relation_version_is_last":true,"title":["Purification and Properties of Intracellular Exo- and Endoinlulinases from Aspergillus niger Strain 12"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-11-14T06:23:19.859473+00:00"}