{"created":"2023-05-15T09:59:07.128712+00:00","id":3203,"links":{},"metadata":{"_buckets":{"deposit":"96299dff-aa15-43f2-af73-025e8cace84e"},"_deposit":{"created_by":5,"id":"3203","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"3203"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00003203","sets":["74","74:46","74:46:349","74:46:349:351"]},"author_link":["16715","1723","1631","16718"],"item_10002_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ブルーベリーヨウ カラ RNA オ コウリツテキ ニ チュウシュツ スル ホウホウ"}]},"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-03-22","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"60","bibliographicPageStart":"57","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"宮崎大学農学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Faculty of Agriculture University of Miyazaki","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The isolation of high-quality RNA from blueberry leaves is difficult due to the presence of many organic acids and polyphenols in the leaves. We established an efficient method for isolating RNA from rabbit-eye blueberry (Vaccinium virgatum Aiton) leaves using a modified cetyl trimethyl ammonium bromide (CTAB) method. More than 200 μg of RNA from 1 g of blueberry leaves was obtained with a HEPES or a MOPS buffer changed from a Tris-HCl buffer. The quality and quantity of the total RNA was sufficient for the cDNA synthesis. We succeeded in the amplification of cDNAs using polymerase chain reaction (PCR).","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"ブルーベリー葉には有機酸やポリフェノールが大量に含まれるため,高品質なRNAを抽出することが難しい.我々は,改良した臭化セチルトリメチルアンモニウム法を用いることにより,ラビットアイブルーベリー (Vaccimium virgatum Aiton) の葉から,RNAを効率的に抽出する手法を確立した. トリス緩衝液をHEPES緩衝液若しくはMOPS緩衝液に変えることで,1gのブルーベリー葉から200μg以上の全RNAを得ることができた.cDNA合成を行うのに十分な質と量であった.また,我々は,RT-PCRによるcDNAの増幅にも成功した.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宮崎大学農学部","subitem_publisher_language":"ja"},{"subitem_publisher":"Faculty of Agriculture, University of Miyazaki","subitem_publisher_language":"en"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00236503","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05446066","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"布施, 拓市","creatorNameLang":"ja"},{"creatorName":"フセ, タクイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西脇, 亜也","creatorNameLang":"ja"},{"creatorName":"ニシワキ, アヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishiwaki, Aya","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"國武, 久登","creatorNameLang":"ja"},{"creatorName":"クニタケ, ヒサト","creatorNameLang":"ja-Kana"},{"creatorName":"Kunitake, Hisato","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Fuse, Takuichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"006_fuse.pdf","filesize":[{"value":"601.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"006_fuse.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/3203/files/006_fuse.pdf"},"version_id":"8edd3366-93ea-417b-93e9-1825fa1a47ab"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CTAB, pH, RT-PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"臭化セチルトリメチルアンモニウム","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An efficient method for the isolation of RNA from blueberry leaves","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An efficient method for the isolation of RNA from blueberry leaves","subitem_title_language":"en"},{"subitem_title":"ブルーベリー葉からRNAを効率的に抽出する方法","subitem_title_language":"ja"}]},"item_type_id":"10002","owner":"5","path":["74","46","349","351"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-05-29"},"publish_date":"2013-05-29","publish_status":"0","recid":"3203","relation_version_is_last":true,"title":["An efficient method for the isolation of RNA from blueberry leaves"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-07-30T03:05:29.643639+00:00"}