{"created":"2023-05-15T09:59:06.637267+00:00","id":3192,"links":{},"metadata":{"_buckets":{"deposit":"34a938d1-bccd-4b8a-8c31-08df7e0fc829"},"_deposit":{"created_by":5,"id":"3192","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"3192"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00003192","sets":["74","74:46","74:46:349","74:46:349:352"]},"author_link":["1787","16648","16649","16650","16651","16652","1788","16655","16656","16657","16658","16659"],"item_10002_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ニクシツ ニ エイキョウ スル タリンツー ノ シゴ ブンカイ ニ オケル カルパインスリー ノ カンヨ"}]},"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-02-24","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"42","bibliographicPageStart":"31","bibliographicVolumeNumber":"58","bibliographic_titles":[{"bibliographic_title":"宮崎大学農学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Faculty of Agriculture University of Miyazaki","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present experiment was conducted to determine which calpain isoforms contribute to the postmortem degradation of the costamere protein talin 2. The chicken pectoralis major (PM) and biceps femoris (BF) muscles gizzard (G) and heart (H) were stored for 4 days at 4℃. Talin 2 in PM and BF were degraded during early postmortem time, but that in G and H were not degraded. While, desmin in PM was degraded faster than the others. The ratio of calpain 1 mRNA level to calpastatin was seemed to correspond to the postmortem degradation of desmin. The ratio of calpain 2 to calpastatin and the ratio of calpain 3 to ribosomal protein large P1 were seemed to correspond to the postmortem degradation of talin 2. Desmin in the isolated myofibrils of the skeletal muscles was degraded with 5 mM Ca2+ and not degraded with leupeptin, the ubiquitous calpain inhibitor. However, talin 2 was degraded with 5 mM Ca2+ and leupeptin. Desmin and talin 2 in the heart were not influenced by 5 mM Ca2+. Furthermore talin 2 was degraded with 1 μM Ca2+ at 4℃, but desmin was not. Therefore, we propose that talin 2 is degraded by calpain 3 during postmortem storage.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"本研究は,コスタメアタンパク質タリン2の死後分解に関わるカルパインアイソフォームを決定するために実施された. ニワトリの胸肉,腿肉,砂のう筋および心筋を4℃で4日間貯蔵した. 胸肉と腿肉のタリン2は死後早期に分解したが,砂のう筋と心筋では分解しなかった. 一方,胸肉のデスミンは他の組織よりも早期に分解した. カルパスタチンに対するカルパイン1の相対的な発現量は,デスミンの死後分解の速度と一致しているように見えた. カルパスタチンに対するカルパイン2の相対的な発現量およびリボゾーマルプロテインラージP1に対するカルパイン3の相対的な発現量は,タリン2の死後分解の速度と一致しているように見えた. 骨格筋から抽出された筋原線維におけるデスミンは,5mMのカルシウムイオンの添加によって分解したが,それはロイペプチンの添加によって阻害された. しかしながら,タリン2は,デスミン同様,5mMのカルシウムイオンの添加で分解したが,ロイペプチンの添加で阻害されなかった. 心筋におけるデスミンとタリン2はともに,5mMのカルシウムイオンに影響を受けなかった. さらに,タリン2は4℃で1μMのカルシウムイオンの添加によっても分解したが,デスミンは分解しなかった. よって,我々は,タリン2が死後の熟成期間中にカルパイン3によって分解されることを提案する.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宮崎大学農学部","subitem_publisher_language":"ja"},{"subitem_publisher":"Faculty of Agriculture, University of Miyazaki","subitem_publisher_language":"en"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00236503","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05446066","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"六車, 三治男","creatorNameLang":"ja"},{"creatorName":"ムグルマ, ミチオ","creatorNameLang":"ja-Kana"},{"creatorName":"Muguruma, Michio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"冨阪, 吉登","creatorNameLang":"ja"},{"creatorName":"トミサカ, ヨシト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森, 名生","creatorNameLang":"ja"},{"creatorName":"モリ, ナオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安岡, 寛子","creatorNameLang":"ja"},{"creatorName":"ヤスオカ, ヒロコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小野, 孝明","creatorNameLang":"ja"},{"creatorName":"オノ, タカアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西, 英二","creatorNameLang":"ja"},{"creatorName":"ニシ, エイジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"河原, 聡","creatorNameLang":"ja"},{"creatorName":"カワハラ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawahara, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Tomisaka, Yoshito","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mori, Nao","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuoka, Hiroko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Takaaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishi, Eiji","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"Vol.58_pp.31-42.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Vol.58_pp.31-42.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/3192/files/Vol.58_pp.31-42.pdf"},"version_id":"cf71db71-1552-4b82-b92e-5adc1cda2a52"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Calpain, Meat quality, Postmortem proteolysis, Talin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"カルパイン, 肉質, 死後分解, タリン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Involvement of Calpain 3 in Postmortem Talin 2 Degradation Influencing Meat Quality","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of Calpain 3 in Postmortem Talin 2 Degradation Influencing Meat Quality","subitem_title_language":"en"},{"subitem_title":"肉質に影響するタリン2の死後分解におけるカルパイン3の関与","subitem_title_language":"ja"}]},"item_type_id":"10002","owner":"5","path":["74","46","349","352"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-06-20"},"publish_date":"2012-06-20","publish_status":"0","recid":"3192","relation_version_is_last":true,"title":["Involvement of Calpain 3 in Postmortem Talin 2 Degradation Influencing Meat Quality"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-07-30T22:53:13.124754+00:00"}