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Effects of calpain and Rho-kinase inhibitors on the acrosome reaction and motility of fowl spermatozoa in vitro
http://hdl.handle.net/10458/723
http://hdl.handle.net/10458/723aefda1d9-3624-4c0f-9378-dae8fd9953c1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa83.pdf (1.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Effects of calpain and Rho-kinase inhibitors on the acrosome reaction and motility of fowl spermatozoa in vitro | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | Acrosome reaction and motility of fowl spermatozoa | |||||
| 言語 | en | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Wishart, G.J.× Katayama, S.× Takano, D.× Maeda, M.× Arakawa, E.× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | At the avian body temperature of 40°C, intact fowl spermatozoa require Ca2+ for the initiation of motility and a combination of both Ca2+ and homogenized inner perivitelline layer (IPVL) together to induce the acrosome reaction. Within the range of 1-100 μmol/l, neither PD 150606, a Ca2+-dependent calpain inhibitor, nor Y-27632, an inhibitor of Ca2+-dependent Rho-kinase, were able to inhibit the acrosome reaction induced by the presence of Ca2+ and IPVL. However, PD 150606, although not Y-27632, was able to inhibit sperm motility initiated by calyculin A, a specific inhibitor of protein phosphatases, which also initiates sperm motility at 40°C. The addition of PD 150606 did not reduce the ATP concentrations of intact spermatozoa, nor the motility of demembranated spermatozoa. Immunoblot analysis of sperm extract using a polyclonal antibody against calpain 12 revealed a crossreacting protein of approximately 80 kDa. These results suggest that Rho-kinase is not involved in the regulation of the acrosome reaction or of motility in fowl spermatozoa. In contrast, calpain appears to be involved in the regulation of flagellar movement, but not in that of the acrosome reaction. Furthermore, it seems that endogenous calpain is present in the cytoplasmic matrix and/or the plasma membrane, but not retained in the axoneme and/or accessory cytoskeletal components. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/rep.1.00588. 2006 Society for Reproduction and Fertility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Reproduction : the official journal of the Society for the Study of Fertility 巻 131, 号 1, p. 71-79, 発行日 2006 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 14701626 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11501251 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
