{"created":"2023-05-15T09:59:02.378455+00:00","id":3115,"links":{},"metadata":{"_buckets":{"deposit":"b0252b39-13a4-4793-8fa4-f13ecebe3229"},"_deposit":{"created_by":5,"id":"3115","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"3115"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00003115","sets":["79","79:42"]},"author_link":["16380","16378","4839","16381","16382"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"141","bibliographicPageStart":"129","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Molecular biology of the cell"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chromatin assembly factor-1 （CAF-1）, a complex consisting of p150, p60, and p48 subunits, is highly conserved from yeast to humans and facilitates nucleosome assembly of newly replicated DNA in vitro. To investigate roles of CAF-1 in vertebrates, we generated two conditional DT40 mutants, respectively, devoid of CAF-1p150 and p60. Depletion of each of these CAF-1 subunits led to delayed S-phase progression concomitant with slow DNA synthesis, followed by accumulation in late S/G2 phase and aberrant mitosis associated with extra centrosomes, and then the final consequence was cell death. We demonstrated that CAF-1 is necessary for rapid nucleosome formation during DNA replication in vivo as well as in vitro. Loss of CAF-1 was not associated with the apparent induction of phosphorylations of S-checkpoint kinases Chk1 and Chk2. To elucidate the precise role of domain（s） in CAF-1p150, functional dissection analyses including rescue assays were preformed. Results showed that the binding abilities of CAF-1p150 with CAF-1p60 and DNA polymerase sliding clamp proliferating cell nuclear antigen （PCNA） but not with heterochromatin protein HP1-gamma are required for cell viability. These observations highlighted the essential role of CAF-1-dependent nucleosome assembly in DNA replication and cell proliferation through its interaction with PCNA.","subitem_description_type":"Abstract"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"17065558","subitem_relation_type_select":"PMID"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2006 by The American Society for Cell Biology"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10591524","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中山, 建男"},{"creatorName":"ナカヤマ, タツオ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakayama, Tatsuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4839","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"髙見, 恭成","creatorNameLang":"ja"},{"creatorName":"Takami, Yasunari","creatorNameLang":"en"},{"creatorName":"高見, 恭成","creatorNameLang":"ja"},{"creatorName":"タカミ, ヤスナリ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"髙見","familyNameLang":"ja"},{"familyName":"Takami","familyNameLang":"en"},{"familyName":"高見","familyNameLang":"ja"},{"familyName":"タカミ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"恭成","givenNameLang":"ja"},{"givenName":"Yasunari","givenNameLang":"en"},{"givenName":"恭成","givenNameLang":"ja"},{"givenName":"ヤスナリ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"16378","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80236356","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80236356"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"髙見, 恭成","creatorNameLang":"ja"},{"creatorName":"Takami, Yasunari","creatorNameLang":"en"},{"creatorName":"高見, 恭成","creatorNameLang":"ja"},{"creatorName":"タカミ, ヤスナリ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"髙見","familyNameLang":"ja"},{"familyName":"Takami","familyNameLang":"en"},{"familyName":"高見","familyNameLang":"ja"},{"familyName":"タカミ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"恭成","givenNameLang":"ja"},{"givenName":"Yasunari","givenNameLang":"en"},{"givenName":"恭成","givenNameLang":"ja"},{"givenName":"ヤスナリ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"16378","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80236356","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80236356"}]},{"creatorNames":[{"creatorName":"Ono, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16380","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Fukagawa, Tatsuo","creatorNameLang":"en"}],"familyNames":[{"familyName":"Fukagawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Tatsuo","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16381","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibahara, 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division in vertebrate cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["79","42"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-12-18"},"publish_date":"2012-12-18","publish_status":"0","recid":"3115","relation_version_is_last":true,"title":["Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2024-07-07T23:43:50.262245+00:00"}