{"created":"2023-05-15T11:25:52.776902+00:00","links":{},"metadata":{"_buckets":{"deposit":"92344e5c-c6c2-417a-be73-9f18afb7c22b"},"_deposit":{"id":"3115.1","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"3115.1"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00003115.1","sets":["79:42"]},"author_link":["4839","16378","16379","16380","16381","16382"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"141","bibliographicPageStart":"129","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Molecular biology of the cell"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chromatin assembly factor-1 （CAF-1）, a complex consisting of p150, p60, and p48 subunits, is highly conserved from yeast to humans and facilitates nucleosome assembly of newly replicated DNA in vitro. To investigate roles of CAF-1 in vertebrates, we generated two conditional DT40 mutants, respectively, devoid of CAF-1p150 and p60. Depletion of each of these CAF-1 subunits led to delayed S-phase progression concomitant with slow DNA synthesis, followed by accumulation in late S/G2 phase and aberrant mitosis associated with extra centrosomes, and then the final consequence was cell death. We demonstrated that CAF-1 is necessary for rapid nucleosome formation during DNA replication in vivo as well as in vitro. Loss of CAF-1 was not associated with the apparent induction of phosphorylations of S-checkpoint kinases Chk1 and Chk2. To elucidate the precise role of domain（s） in CAF-1p150, functional dissection analyses including rescue assays were preformed. Results showed that the binding abilities of CAF-1p150 with CAF-1p60 and DNA polymerase sliding clamp proliferating cell nuclear antigen （PCNA） but not with heterochromatin protein HP1-gamma are required for cell viability. These observations highlighted the essential role of CAF-1-dependent nucleosome assembly in DNA replication and cell proliferation through its interaction with PCNA.","subitem_description_type":"Abstract"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"17065558","subitem_relation_type_select":"PMID"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2006 by The American Society for Cell Biology"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10591524","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中山, 建男"},{"creatorName":"ナカヤマ, タツオ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakayama, Tatsuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高見, 恭成"}],"nameIdentifiers":[{"nameIdentifier":"16378","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80236356","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80236356"}]},{"creatorNames":[{"creatorName":"Takami, Yasunari","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16379","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16380","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukagawa, Tatsuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16381","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibahara, Kei-Ichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16382","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"nakayama.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nakayama.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/3115.1/files/nakayama.pdf"},"version_id":"5e374156-9aea-4dad-938b-60703cc4fb8c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"2","path":["42"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-12-18"},"publish_date":"2012-12-18","publish_status":"0","recid":"3115.1","relation_version_is_last":true,"title":["Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-07-24T05:53:30.894778+00:00"}