{"created":"2023-05-15T09:58:59.929123+00:00","id":3077,"links":{},"metadata":{"_buckets":{"deposit":"97a05431-40c8-4e1d-87d3-fdf86883f7b3"},"_deposit":{"created_by":5,"id":"3077","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"3077"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00003077","sets":["72","72:40"]},"author_link":["7970"],"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(1)閉塞性黄疸ラットモデルでのトランスポーターの発現:今回の科研のテーマの1つである総胆管結紮閉塞性黄疸モデルを用い、胆管結紮後3日から28日までのタイムコースをとり血液、肝臓を採取した。胆管閉塞後、排泄系トランスポーターmrp2の発現は低下し、mrp3の発現は上昇した。これらの変化は28日後も持続していた。取り込み系トランスポーターoatp, ntcpの発現は胆管結紮後早期に低下し、28日後も低下していた。毛細胆管への排出に関わるmdr2の発現に変化はなかった。黄疸期間により、これらトランスポーターのmRNAの発現変化が異なることが示唆され、現在蛋白発現を検討中である。\n(2)大量肝切除ラットモデルでの肝予備能:70%肝切除と急性肝不全モデルである90%肝切除をラットで行い、胆汁流量、胆汁酸排泄、トランスポーターの発現を検討した。90%肝切除群においてmrp2の発現は術後低下し、mrp3、bsepの発現は術後上昇したのに対し、70%肝切除後には逆の変動を示した。取り込み系トランスポーターのntcp,oatpの発現は両群とも術後低下し、差は認めなかった。70%肝切除群と90%肝切除群の間で排泄系トランスポーターmrp2, mrp3の発現変化が異なり、術後肝不全発症の早期指標の一つとなる可能性が示唆され、現在更に検討している。90%肝切除後には胆汁流量、胆汁中胆汁酸排泄は有意に低下しており、現在論文として投稿中である。\n(3)門脈塞栓後および虚血・再潅流後の肝再生と肝障害の分子細胞学的解析:門脈結紮モデルを作成し、肝再生葉と萎縮葉のサイクリンD1の発現の違いが、肝再生・萎縮に関係することを報告した。(研究発表1)また、虚血・再潅流肝障害の機序の一部を報告した(発表2)。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"1. The differences in the expression of the organic anion (bile acid,bilirubin) transporters depending on the duration of bile duct ligation have been examined in rats.  Blood and liver tisues during the period from 3 to 28 days after the ligation were obtained.  The excretion transporters,the mRNA expressions of mrp2 decreased while those of mrp3 increased from 3 days until 28 days after the ligation.  The uptake transporters,ntcp and oatp decreased until 28 days after the ligation,while the mdr2 did not change after the ligation.  We are now examining the expression of these protein levels.\n2. The differences in hepatic reserve function and parameters to predict the liver failure in  the early stage after extended hepatectomy were examined in rats.We determined the bile flow,biliary bile acid secretion and the mRNA expressions of bile acid transporters after 70 % or 90 % hepatectomy in rats.  The bile flow and bile acid secretion both decreased significantly after the 90 % hepatectomy, which is a model for acute liver failure.  Biliary bile acid excretion and the expression of mrp2 decreased,while the expressions of mrp3 and bsep increased after 90 % hepatectomy.  In contrast, the expression of mrp2 increased, while the expressions of mrp3 and bsep decreased after 70 % hepatectomy.  The expressions of mrp2 and mrp3 was differently regulated after 70 % and 90 % hepatectomy.  We suspected that expressions of mrp2 and mrp3 might be able to predict the liver failure after extended hepatectomy.\n3. We examined the expression of growth factors, transcription factors, and cell cycle regulators in response to regeneration after a two-third hepatectomy and portal vein branch ligation.  We reported that the mRNA expression of Cyclin D1 decreased in the atrophying and increased in the proliferating lobes after portal vein branch ligation and after hepatectomy in rats (ref. 1).  We also reported the mechanisms of liver damage after hepatic ischemia and reperfusion in rats (ref. 2).","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成15年度~平成16年度科学研究費補助金(基盤研究(c)(2))研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1016/j.jss.2003.11.020"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.jss.2003.11.020","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1016/j.jss.2003.07.004"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.jss.2003.07.004","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1016/S0022-4804(03)00360-3"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/S0022-4804(03)00360-3","subitem_relation_type_select":"DOI"}}]},"item_10007_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"学術雑誌掲載論文の抜き刷りは、出版社に著作権許諾が得られていなのため未掲載","subitem_rights_language":"ja"}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"tidiiwa_H17.pdf","filesize":[{"value":"194.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tidiiwa_H17.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/3077/files/tidiiwa_H17.pdf"},"version_id":"587c1f22-b5a1-41bc-b851-e044e2add4f1"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"15591417gaiyo.pdf","filesize":[{"value":"220.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"15591417gaiyo.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/3077/files/15591417gaiyo.pdf"},"version_id":"df64259f-4e02-412e-97e8-2ed0c17bee16"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bile acid transporter, Hepatectomy, Liver failure, Obstructive jaundice, Portal vein ligation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"トランスポーター, 大量肝切除, 肝不全, 門脈結紮, 閉塞性黄疸","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"千々岩, 一男","creatorNameLang":"ja"},{"creatorName":"チヂイワ, カズオ","creatorNameLang":"ja-Kana"},{"creatorName":"Chijiiwa, Kazuo","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"肝予備能把握法の確立と肝切除後残存肝機能の分子生物学的解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"肝予備能把握法の確立と肝切除後残存肝機能の分子生物学的解析","subitem_title_language":"ja"},{"subitem_title":"Establishment of the hepatic reserve and molecular biological analyses of liver function after hepatectomy","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"5","path":["72","40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-06-14"},"publish_date":"2013-06-14","publish_status":"0","recid":"3077","relation_version_is_last":true,"title":["肝予備能把握法の確立と肝切除後残存肝機能の分子生物学的解析"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2024-01-30T04:26:57.933166+00:00"}