@article{oai:miyazaki-u.repo.nii.ac.jp:00000264, author = {酒井, 洋樹 and 中野, 裕子 and Yamaguchi, Ryoji and 山口, 良二 and 米丸, 加余子 and 柳井, 徳磨 and 柵木, 利昭 and Sakai, Hiroki and Nakano, Hiroko and Yonemaru, Kayoko and Yanai, Tokuma}, issue = {6}, journal = {The journal of veterinary medical science}, month = {Jun}, note = {A new canine cell line, named CCT, was established from the cutaneous malignant histiocytosis in a 4-year-old male Borzoi. CCT proliferated with loose adherence and doubling time was approximately 30 hr. When co-cultured with latex beads, CCT phagocytized beads vigorously. Lysozyme and vimentin were positive by immunostaining, and non-specific esterase and acid phosphatase were positive by cytochemical staining. These features indicated the cells had a histiocytic nature. Furthermore, by subcutaneous injection to nude mice CCT could successfully form tumors with the morphological and immunohistochemical features similar to the original tumor., 4歳雄ボルゾイの皮膚の悪性組織球症より新しい犬の細胞株CCTを樹立した. CCTは緩く接着しつつ増殖し,倍加時間は約30時間であった.ラテックスビーズと混合培養によりCCTはビーズを旺盛に貧食し,免疫染色でビメンチンおよびリゾチーム,細胞化学染色で非特異的エステラーゼおよび酸性ホスファターゼが陽性を呈し,組織球の性質を示した.さらに,ヌードマウス皮下接種により,原発腫瘍と同様の特徴を有する腫瘍を形成した.}, pages = {731--735}, title = {Establishment of a new canine cell line (CCT) originated from a cutaneous malignant histiocytosis}, volume = {65}, year = {2003}, yomi = {サカイ, ヒロキ and ナカノ, ヒロコ and ヤマグチ, リョウジ and ヨネマル, カヨコ and ヤナイ, トクマ and マセギ, トシアキ} }