@article{oai:miyazaki-u.repo.nii.ac.jp:00000262,
 author = {川畑, 敦 and Kawabata, Atsushi and 山本, 香織 and 内田, 和幸 and Uchida, Kazuyuki and Yamaguchi, Ryoji and 山口, 良二 and 林, 俊春 and Hayashi, Toshiharu and 立山, 晉 and Tateyama, Susumu and 川畑, 敦 and Kawabata, Atsushi and Yamamoto, Kaori and LAN, Nguyen Thi and 林, 俊春 and Hayashi, Toshiharu and LAN, Nguyen Thi},
 issue = {10},
 journal = {The journal of veterinary medical science},
 month = {Oct},
 note = {A cell line, MCO-Y4, was established from a mammary gland osteosarcoma of a 16-year-old female mongrel dog. Histopathologically the tumor was composed of osteoblastic cells with an osteoid meshwork and chondroid matrix. The mean doubling time of the cells at the 93rd passage was 32.39±4.66 hr. Immunohistochemically, the osteoblastic and chondroblastic cells were positive for bone morphogenetic protein （BMP）-2/4 and BMP receptor （BMPR） II. The cultured cells were spindle in shape during the growth and the confluent phases. No tumor matrix was detected in the culture dish by alcian blue staining or von-Kossa silver impregnation. MCO-Y4 cells on the chamber slides showed intense immunoreactivity for BMP-2/4 and BMPR II. Noggin, an antagonist for BMP-2/4, showed the growth inhibition on MCO-Y4 cells. In addition, fibronectin might be potential for stimulating growth of MCO-Y4 cells. When transplanted into severe combined immunodeficiency mice, the cells formed tumors consisting of solid proliferation of osteoblastic and fibroblastic cells with woven-bone trabeculae. These tumor cells were intensely positive for BMP-2/4 and BMPR II. Our results suggested that the cell line might be useful for studying the role of BMPs in canine osteosarcoma and the mechanism of ossification., 16歳雑種犬の乳腺骨肉腫より,MCO-Y4細胞を樹立した.病理組織学的に原発腫瘍は,類骨,軟骨の形成を伴い,骨芽様及び軟骨芽細胞で構成され,免疫組織化学的に,両細胞型とも骨形成タンパク（BMP）-2/4及びBMP受容体（BMPR）IIに陽性であった.培養細胞は,増殖及び休止期共に紡錘型を呈して平均倍加時間は,32.39±4.66時間であった.培養下では基質産生はアルシアンブルー染色,フォンコッサ反応で確認できなかった.培養MCO-Y4細胞は,免疫組織化学的にBMP-2/4及びBMPR IIに強陽性であった.BMP-2/4の拮抗物質であるノギンは,MCO-Y4細胞増殖を抑制し,フィブロネクティンは,MCO-Y4細胞増殖を促進した.重症複合型不全マウスに接種後形成された腫瘍は,骨梁の形成を伴い,骨芽様及び線維芽樣細胞により構成され,両細胞型共に,免疫組織化学的にBMP-2/4及びBMPR IIに強陽性であった.以上の結果は,MCO-Y4がイヌ骨肉腫又は骨化におけるBMPsの役割を研究するのに有用であることを示唆するものである.},
 pages = {1047--1053},
 title = {Establishment and characterization of a cell line, MCO-Y4, derived from canine mammary gland osteosarcoma(Pathology)},
 volume = {68},
 year = {2006},
 yomi = {カワバタ, アツシ and ヤマモト, カオリ and ウチダ, カズユキ and ヤマグチ, リョウジ and ハヤシ, トシハル and タテヤマ, ススム and カワバタ, アツシ and ハヤシ, トシハル}
}