@article{oai:miyazaki-u.repo.nii.ac.jp:00000256,
 author = {川畑, 敦 and Kawabata, Atsushi and 岡野, 久美子 and 内田, 和幸 and Uchida, Kazuyuki and Yamaguchi, Ryoji and 山口, 良二 and 林, 俊春 and Hayashi, Toshiharu and 立山, 晉 and Tateyama, Susumu and 川畑, 敦 and Kawabata, Atsushi and Okano, Kumiko and 林, 俊春 and Hayashi, Toshiharu},
 issue = {11},
 journal = {The journal of veterinary medical science},
 month = {Nov},
 note = {To compare the roles of chondromodulin-I （ChM-I） and bone morphogenetic protein-6 （BMP-6） in ectopic mesenchymal tissue formation in canine mammary gland tumors, 33 tumors and 2 normal mammary glands were examined. Immunohistochemical analysis revealed co-expression of ChM-I and BMP-6 in canine mammary tumors. In mixed tumors, newly formed woven bone with ossified cartilage matrix was observed in 4/9 cases. The osteoblasts lining the woven bone showed moderate immunoreactivity to ChM-I and BMP-6. Almost all chondrocytes and proliferative myoepithelial cells within the basement membrane showed intense immunoreactivity to both, and the myoepithelial cells adjacent to the mature cartilage showed the most intense immunoreactivity. The immunoreactivity to ChM-I and BMP-6 of the interstitial myoepithelial cells in the myxomatous stroma varied in each focus of mixed tumors. Similar findings were found in complex adenomas. In simple adenomas, hyperplasic myoepithelial cells within the basement membrane showed moderate immunoreactivity to both markers. Western blot analysis detected a 25kDa band of ChM-I in fresh tissue samples from three mixed tumors. Our results support the hypothesis that proliferating myoepithelial cells with ChM-I and BMP-6 expression play important roles in mesenchymal metaplasia in canine mammary tumors., イヌ乳腺腫瘍内の間葉系組織形成と筋上皮細胞におけるChM-I及びBMP-6の関連性を検討するため, イヌ乳腺腫瘍33例及び正常乳腺組織2例について免疫組織学的に検索した.また混合腫瘍3例の凍結材料についてはウエスタンブロット法（WB法）を実施し, ChM-Iの発現を検索した.混合腫瘍では, ほぼ全ての軟骨細胞, 基底膜内筋上皮細胞及び成熟軟骨組織に接する筋上皮細胞にChM-IとBMP-6の強い発現が認められた.粘液腫状間質の筋上皮細胞におけるChM-IとBMP-6の発現は, 部位により様々であった.複合型乳腺腫では, 基底膜内筋上皮細胞でChM-Iの強い発現が認められ, 間質の筋上皮細胞については混合腫瘍と同様の所見であった.単純型乳腺腫では, 基底膜内筋上皮細胞にChM-IとBMP-6の比較的強い発現が認められた.WB法では, 3例ともChM-Iに特異的な25 kDaの陽性バンドが認められた.以上より, 筋上皮細胞におけるChM-I及びBMP-6の発現は, イヌ乳腺腫瘍の間葉組織化生に関連することが示唆された.},
 pages = {1097--1102},
 title = {Co-localization of chondromodulin-I (ChM-I) and bone morphogenetic protein-6 (BMP-6) in myoepithelial cells of canine mammary tumors},
 volume = {67},
 year = {2005},
 yomi = {カワバタ, アツシ and オカノ, クミコ and ウチダ, カズユキ and ヤマグチ, リョウジ and ハヤシ, トシハル and タテヤマ, ススム and カワバタ, アツシ and ハヤシ, トシハル}
}