{"created":"2023-05-15T09:56:37.442702+00:00","id":255,"links":{},"metadata":{"_buckets":{"deposit":"275bbde0-a335-4850-b1ed-1a8c475f0e41"},"_deposit":{"created_by":5,"id":"255","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"255"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000255","sets":["74","74:26"]},"author_link":["966","964","21409","968","939","937","940","962"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-05-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"495","bibliographicPageStart":"491","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"The journal of veterinary medical science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To know growth profiles of canine distemper virus （CDV） on Vero cells stably expressing canine signaling lymphocyte activation molecule （Vero-DogSLAMtag; Vero-DST cells）, the propagation of three strains of CDV was tested in Vero-DST cells in comparison with parental Vero cells. Strain MD77 could grow well in both cell lines, but demonstrated no syncytium formation or indistinguishable rounding cytopathic effects （CPE） in Vero cells. Strains Onderstepoort and KDK-1 also grew well in Vero-DST cells with apparent syncytium CPE, while they grew less or no efficiently, respectively, in Vero cells. All three CDV strains demonstrated the peak titers, in Vero-DST cells before reaching to an extensive CPE and drastic decrease of titers at/after full CPE. Immunohistochemistry revealed that viral antigens of all CDV strains were found exclusively in the syncytia in Vero-DST cells, while in Vero cells, viral antigen was identified in their single cells for strain MD77 but none for other strains. Thus, every strain of CDV could grow well in Vero-DST cells and behaved differently against Vero cells. These results would be of practical value for workers of CDV because 1） In Vero-DST cells, by observation of distinct syncytium CPE, the highest titer or the best growth of virus could be identified; 2） In Vero cells, various CDV strains could be readily classified after propagation in Vero-DST cells.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"Canine signaling lymphocyte activation molecule発現Vero（Vero-DogSLAMtag; Vero-DST）細胞での増殖の特徴を知るために, 3株のイヌジステンパーウイルス（CDV）の増殖を親Vero細胞における増殖と比較して調べた.MD77株は両細胞でよく増殖したが, Vero細胞では合胞体不形成又は円形化CPEを示した.OnderstepoortとKDK-1は共にVero-DST細胞で明瞭な合胞体形成CPEを示しよく増殖したが, Vero細胞ではそれぞれ増殖が低度かほとんど増殖しなかった.3株すべてCPEが全体に拡がる前にVero-DST細胞でウイルス力価はピークになり, 全面に拡がった時に又はその後急速に激減した.免疫組織化学的に感染Vero-DST細胞ではすべての株で合胞体にウイルス抗原が存在し, MD77株感染Vero細胞では単独細胞に抗原がみられた.このようにすべてのVero細胞では別々の性状を示すCDV3株はVero-DST細胞でよく増殖したが, 合胞体形成及び増殖速度に違いがみられた.これらの結果は1）Vero-DST細胞で明瞭な合胞体CPEを観察することによってウイルスの最高値又は最高増殖が判断可能, 2）Vero-DST細胞でCDV増殖後, Vero細胞でMD77, Onderstepoort, KDK-1タイプに分類可能でCDV研究において実用面で有用であると考えられた.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Veterinary Science","subitem_publisher_language":"en"},{"subitem_publisher":"日本獣医学会","subitem_publisher_language":"ja"}]},"item_10001_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://ci.nii.ac.jp/naid/110003963757/"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本獣医学会","subitem_rights_language":"ja"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである","subitem_rights_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10796138","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09167250","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Lan, Nguyen Thi","creatorNameLang":"en"},{"creatorName":"ラン, グエン ティ","creatorNameLang":"ja"},{"creatorName":"ラン, グエン ティ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Lan","familyNameLang":"en"},{"familyName":"ラン","familyNameLang":"ja"},{"familyName":"ラン","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Nguyen Thi","givenNameLang":"en"},{"givenName":"グエン ティ","givenNameLang":"ja"},{"givenName":"グエン ティ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"21409","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Yamaguchi, Ryoji","creatorNameLang":"en"},{"creatorName":"山口, 良二","creatorNameLang":"ja"},{"creatorName":"ヤマグチ, リョウジ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Yamaguchi","familyNameLang":"en"},{"familyName":"山口","familyNameLang":"ja"},{"familyName":"ヤマグチ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Ryoji","givenNameLang":"en"},{"givenName":"良二","givenNameLang":"ja"},{"givenName":"リョウジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"940","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90150169","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90150169"}]},{"creatorNames":[{"creatorName":"甲斐, 一成","creatorNameLang":"ja"},{"creatorName":"カイ, カズシゲ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"962","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"内田, 和幸","creatorNameLang":"ja"},{"creatorName":"ウチダ, カズユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Uchida, Kazuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"939","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤, 篤","creatorNameLang":"ja"},{"creatorName":"カトウ, アツシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"964","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"立山, 晉","creatorNameLang":"ja"},{"creatorName":"タテヤマ, ススム","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"937","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"LAN, Nguyen Thi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"966","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kai, Kazushige","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"968","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"jvms67_5_491.pdf","filesize":[{"value":"809.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JVMS67(5)491.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/255/files/jvms67_5_491.pdf"},"version_id":"597ee20f-5960-46cb-8b89-07c1349f33a9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CDV, CPE, growth properties, Vero cell, Vero-DST cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The growth profiles of three types of canine distemper virus on vero cells expressing canine signaling lymphocyte activation molecule","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The growth profiles of three types of canine distemper virus on vero cells expressing canine signaling lymphocyte activation molecule","subitem_title_language":"en"},{"subitem_title":"Canine Signaling Lymphocyte Activation Molecule発現Vero細胞における3タイプのイヌジステンパーウイルス株の増殖特徴","subitem_title_language":"ja"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-08"},"publish_date":"2008-02-08","publish_status":"0","recid":"255","relation_version_is_last":true,"title":["The growth profiles of three types of canine distemper virus on vero cells expressing canine signaling lymphocyte activation molecule"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-11-08T07:20:41.546098+00:00"}