{"created":"2023-07-29T13:19:27.786626+00:00","links":{},"metadata":{"_buckets":{"deposit":"237f7e0f-5b2c-4f44-982a-8cf909c1faed"},"_deposit":{"created_by":9,"id":"253.2","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"253.2"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000253.2","sets":["74","74:26"]},"author_link":["936","937","938","939","940","941","942","943","945"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-11-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"1219","bibliographicPageStart":"1217","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"The journal of veterinary medical science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The nucleptide sequence of canine α-lactalbumin cDNA from canine mammary tissue was determined by polymerase chain reaction with degenerate primers. A 742 base pairs nucleotide sequence cloned was similar to the size of mRNA in Northern blot analysis. The cDNA encodes 142 amino acid residues containing the conserved sequence motif of -lactalbumin, demonstrating the highest homology with pig（73% identity-82% similarity）among the known amino acid sequences of α-lactalbumin. The canine cDNA also showed 71% identity-78% similarity with human, 58-73% with mouse, 60-74% with rat, 67-77% with goat, 66-77% with cattle, and 67-76% with sheep, respectively.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"犬の乳腺組織由来cDNAライブラリーを用いてα-lactalbuminをコードするcDNAのクローニングを行った.degenerative oligo primer法及び5', 3'RACE法により742塩基対のcDNAをクローニングし、ノザンブロット法より完全長であることが示唆された.同cDNAのは142残基のアミノ酸をコードしており, アミノ酸レベルにおいて人, マウス, ラット, 山羊, 牛、羊のα-lactalbuminとそれぞれ71%, 58%, 60%, 67%, 66%, 67%の相同性を示し哺乳類間でよく保存されていることが確認された.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Veterinary Science","subitem_publisher_language":"en"},{"subitem_publisher":"日本獣医学会","subitem_publisher_language":"ja"}]},"item_10001_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://ci.nii.ac.jp/naid/110003920498/"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本獣医学会","subitem_rights_language":"ja"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである","subitem_rights_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10796138","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09167250","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡邊, 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リョウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamaguchi, Ryoji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"940","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90150169","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90150169"}]},{"creatorNames":[{"creatorName":"清水, 孜","creatorNameLang":"ja"},{"creatorName":"シミズ, ツトム","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"941","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"管野, 純夫","creatorNameLang":"ja"},{"creatorName":"スガノ, スミオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"942","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Manabu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"943","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Togashi, Takushi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"945","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"jvms62_11_1217.pdf","filesize":[{"value":"382.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JVMS62(11)1217.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/253/files/jvms62_11_1217.pdf"},"version_id":"f7700b05-ca01-4bee-a035-78f9d64d52d9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"canine, degenerate oligo primer, α-lactalbumin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of Canine α-Lactalbumin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of Canine α-Lactalbumin","subitem_title_language":"en"},{"subitem_title":"犬α-lactalbumin cDNAのクローニング","subitem_title_language":"ja"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-08"},"publish_date":"2008-02-08","publish_status":"0","recid":"253.2","relation_version_is_last":true,"title":["Identification of Canine α-Lactalbumin"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-07-29T13:19:30.581892+00:00"}