{"created":"2023-05-15T09:56:37.244681+00:00","id":252,"links":{},"metadata":{"_buckets":{"deposit":"b1d8a171-9bbf-4579-bd97-ce959cd6a360"},"_deposit":{"created_by":5,"id":"252","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"252"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000252","sets":["74","74:26"]},"author_link":["934","935","932"],"control_number":"252","item_10001_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Demembranated models of fowl spermatozoa","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"270","bibliographicPageStart":"263","bibliographicVolumeNumber":"86","bibliographic_titles":[{"bibliographic_title":"Journal of reproduction and fertility","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Triton X-100 at a concentration of 0.1% in the extraction medium was optimal for demembranating fowl spermatozoa. The most suitable conditions for reactivation were obtained when a medium composed of 0.5 mM-ATP, 25 mM-potassium glutamate, 10-7 M-CaCl2, 20 mM-Tris-HCl (pH 7.9), 1 mM-MgSO4, l mM-dithiothreitol and 0.2 M-sucrose was used. More than 60% motile spermatozoa were obtained under these conditions. The addition of 1 or 10 μM-cAMP did not appreciably affect motility. Intact and demembranated spermatozoa were immotile at 40°C, whilst at 30°C motility was restored.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. 1989 Society for Reproduction and Fertility.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00705952","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00224251","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Ashizawa, Koji","creatorNameLang":"en"},{"creatorName":"芦沢, 幸二","creatorNameLang":"ja"},{"creatorName":"アシザワ, コウジ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Y.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okauchi, K.","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ashizawa30.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ashizawa30.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/252/files/ashizawa30.pdf"},"version_id":"18da28e1-0b5d-4088-bb8f-d551a517a808"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm, spermatozoa, motility, demembranated models, temperature, fowl","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Flagellar movement in demembranated preparations of ejaculated fowl spermatozoa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Flagellar movement in demembranated preparations of ejaculated fowl spermatozoa","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-10-10"},"publish_date":"2007-10-10","publish_status":"0","recid":"252","relation_version_is_last":true,"title":["Flagellar movement in demembranated preparations of ejaculated fowl spermatozoa"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-10-09T05:34:18.422508+00:00"}