{"created":"2023-05-15T12:33:57.446333+00:00","links":{},"metadata":{"_buckets":{"deposit":"f157a867-d536-4f78-8864-1eb800a3c99a"},"_deposit":{"id":"2102.1","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2102.1"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00002102.1","sets":["85:33"]},"author_link":["2632"],"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"カビの一種Aureobasidium pullulans var. melanigenum ATCC 20524株は活性の至適pHが2.0である好酸性キシラナーゼを産生する。今年度は、メタノール資化性酵母Pichia pastoris GS115株による本酵素の発現と分泌を目的とした。キシランを炭素源とする液体培地で増殖させた本キシラナーゼ生産菌株の培養菌体からmRNAを調製した。このmRNAから、RT-PCRによりキシラナーゼをコードするcDNAを合成した。シグナル配列がそれぞれ本酵素自身のもの、Saccharomyces cerevisiae のα-ファクター、P.pastoris のPHO1となるようにキシラナーゼcDNAを染色体組込み型の3種の分泌発現ベクターpPIC3.5、pPIC9、およびpHIL-S1(インビトロ-ゲン社)に挿入し、P.pastorisを形質転換した。これら形質転換体を30℃ でメタノールにより遺伝子の発現を誘導しながら液体培養で増殖させた結果、細胞外にキシラナーゼを効率よく分泌した。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ohta2-chiiki-2003.pdf","filesize":[{"value":"782.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ohta2-chiiki-2003.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/2102.1/files/ohta2-chiiki-2003.pdf"},"version_id":"e45fd1e3-0a76-44e6-b550-f52f1193f59d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"太田, 一良","creatorNameLang":"ja"},{"creatorName":"オオタ, カズヨシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2632","nameIdentifierScheme":"WEKO"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"多糖キシランを分解する遺伝子組換え酵母","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"多糖キシランを分解する遺伝子組換え酵母","subitem_title_language":"ja"}]},"item_type_id":"10007","owner":"2","path":["33"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-12-22"},"publish_date":"2008-12-22","publish_status":"0","recid":"2102.1","relation_version_is_last":true,"title":["多糖キシランを分解する遺伝子組換え酵母"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-07-13T04:04:09.395821+00:00"}