| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-03-23 |
| タイトル |
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タイトル |
Screening of Bradyrhizobium ottawaense with High N2O-reducing Activity from Soybean Nodules in Japan |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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言語 |
en |
|
キーワード |
N2O reduction |
| キーワード |
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言語 |
en |
|
キーワード |
bradyrhizobia |
| キーワード |
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|
言語 |
en |
|
キーワード |
nitrous oxide |
| キーワード |
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言語 |
en |
|
キーワード |
nosZ |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Itakura, Manabu
Kakizaki, Kaori
Suzuki, Atsuo
Okubo, Satoshi
Kato, Hiromi
Sugawara, Masayuki
佐伯, 雄一
WEKO
4564
e-Rad_Researcher
50295200
| ja |
佐伯, 雄一
宮崎大学
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| ja-Kana |
サエキ, ユウイチ
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| en |
Saeki, Yuichi
University of Miyazaki
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Search repository
Minamisawa, Kiwamu
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Bradyrhizobium ottawaense has prospects as an environmentally friendly inoculant for soybean farming because of its higher N2O reductase (N2OR) activity than that of B. diazoefficiens. To examine high N2O-reducing B. ottawaense, we performed a PCR anal-ysis of nosZ genes in 8,640 soybean nodules from 68 fields in Japan. Of 384 PCR-positive nodules, we obtained 90 isolates of bradyrhizobia with B. ottawaense-type nosZ, derived exclusively from 18 fields in Gunma and Osaka prefectures. Of 77 monophyletic isolates, 73 had significantly higher N2OR activity than B. diazoefficiens USDA110. Another 13 isolates from Osaka were phylogenetically placed outside of the B. ottawaense clade with B. liaoningense or B. betae, 8 of which also exhibited significantly higher N2OR activity than B. diazoefficiens USDA110. An anal-ysis of nopP gene sequences revealed amino acid sequence variations in the NopP effector protein among these high N2O-reducing isolates, with the NopPUSDA122 type being one of the variations identified. The NopP-mediated symbiotic incompatibility of soybean host plants may eliminate nodulation by indigenous bradyrhizobia and facilitate inoculant nodulation to reduce N2O emissions. Therefore, 90 isolates and their observed NopP types are potentially important resources for N2O mitigation. Furthermore, the dense geographical map of Bradyrhizobium species based on Internal Transcribed Spacer-Restriction Fragment Length Polymorphisms (ITS-RFLP) of the 16S-23S rRNA gene from 8,640 nodules revealed the recent northward expansion of B. elkanii to central Japan potentially due to global warming. This change in indigenous soybean bradyrhizobia is important for application strategies of bradyrhizobial inoculants under field conditions. |
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言語 |
en |
| 書誌情報 |
en : Microbes and environments
巻 41,
号 1,
p. ME25062,
発行日 2026
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| 出版者 |
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出版者 |
Japanese Society of Microbial Ecology |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
13474405 |
| DOI |
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関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1264/jsme2.ME25062 |
| 権利 |
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権利情報 |
© 2026 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
Fulltext (941 KB)
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