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A competitive fluorescence immunoassay based on intermolecular quenching using an N-terminally fluorescent-labeled IgG antibody
http://hdl.handle.net/10458/0002002374
http://hdl.handle.net/10458/00020023742a9f5823-89a0-42f6-82be-45659ed6b953
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AuthorManuscript (1.5 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2026-03-09 | |||||||||
| タイトル | ||||||||||
| タイトル | A competitive fluorescence immunoassay based on intermolecular quenching using an N-terminally fluorescent-labeled IgG antibody | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| キーワード | Antibody | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| キーワード | FRET | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| キーワード | Immunoassay | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| キーワード | Quencher | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| キーワード | Thyroxine | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ | journal article | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| 著者 |
福永, 圭佑
× 福永, 圭佑× Watanabe, Takayoshi
× Hohsaka, Takahiro
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The development of antibody-based fluorescent sensors relying on tryptophan-mediated quenching, such as Quenchbody (Q-body), often exhibits limited fluorescence responses because dye quenching depends on the location of tryptophan residues within the antibody. Here, we developed a competitive fluorescence immunoassay, termed an intermolecular Quenchbody (iQ-body), that utilizes intermolecular Förster resonance energy transfer (FRET) between an N-terminally fluorescent-labeled IgG antibody and an antigen-quencher conjugate. Anti-thyroxine IgG antibody (clone 6901 SPTN-5) showed minimal fluorescence changes upon antigen binding, despite N-terminal labeling with TAMRA. In contrast, the addition of an antigen-quencher conjugate (QSY9-X-T3) effectively quenched the TAMRA fluorescence via intermolecular FRET. Subsequent competitive displacement by thyroxine (T4) resulted in concentration-dependent fluorescence recovery. The iQ-body strategy provides a simple approach for constructing competitive fluorescence immunoassays for small-molecule targets using publicly available IgG antibodies. | |||||||||
| 言語 | en | |||||||||
| 書誌情報 |
en : Biochemical and biophysical research communications 巻 808, p. 153449, 発行日 2026-04-09 |
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| 出版者 | ||||||||||
| 出版者 | Elsevier BV | |||||||||
| 言語 | en | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | EISSN | |||||||||
| 収録物識別子 | 10902104 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isVersionOf | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.1016/j.bbrc.2026.153449 | |||||||||
| 権利 | ||||||||||
| 権利情報 | © 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies. | |||||||||
| 言語 | en | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | AM | |||||||||
