WEKO3
アイテム
FK506 causes pain by upregulating NaV1.7 channels in the spinal dorsal root ganglia of NaV1.7-ChR2 mice
http://hdl.handle.net/10458/0002002262
http://hdl.handle.net/10458/0002002262a50b8e50-adca-471e-85bb-49a0fcc37409
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Fulltext (958 KB)
|
.png)
|
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2026-02-03 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | FK506 causes pain by upregulating NaV1.7 channels in the spinal dorsal root ganglia of NaV1.7-ChR2 mice | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| キーワード | FK506 | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| キーワード | NaV1.7 | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| キーワード | calcineurin inhibitor-induced pain syndrome | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| キーワード | dorsal root ganglion | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| キーワード | optogenetics | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| キーワード | tacrolimus | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||
| 著者 |
丸田, 豊明
× 丸田, 豊明
WEKO
34465
× Shiraishi, Seiji
× 興梠, 聡志
WEKO
35157
× Kurogi, Mio
× Hirata, Naoyuki
|
|||||||||||||||||||||||
| 抄録 | ||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||
| 内容記述 | Calcineurin inhibitors, including tacrolimus (FK506), are used as immunosuppressive agents and can cause unexplained calcineurin inhibitor-induced pain syndrome (CIPS). We investigated how FK506 affects the expression of NaV1.7, a voltage-gated Na+ channel implicated in pain perception that is upregulated in dorsal root ganglion (DRG) neurons in several pain disorders. We generated a model of FK506-induced pain by administering FK506 to NaV1.7-ChR2 mice, which exhibit light-responsive pain. To evaluate nociceptive responses, paw withdrawal threshold (PWT) was measured using the von Frey test. The optogenetic place aversion (OPA) and light irradiation paw withdrawal tests were also performed. On the 11th day of initial injection, DRGs were dissected from mice under anesthesia and analyzed for NaV1.7 expression using quantitative reverse transcription PCR (RT-qPCR). PWT was also measured for mice that received the selective NaV1.7 inhibitor or vehicle. PWT was lower in FK506-treated mice than in those administered the vehicle on the 8th and 12th days after initial injection (P < 0.05). Mechanical hypersensitivity was reversible and peaked at around 10 days after FK506 administration. OPA and light irradiation paw withdrawal test results corroborated the hypersensitivity to light-responsivity. NaV1.7 mRNA levels in DRG were higher in FK506-treated mice than in those administered the vehicle on the 11th day (P < 0.05). A selective NaV1.7 inhibitor reversed FK506-induced pain. Increased NaV1.7 expression in DRG neurons may be responsible for FK506-induced peripheral neuropathy. Our findings suggest that endogenous calcineurin regulates NaV1.7 expression. Thus, selective NaV1.7 inhibition could be a potential therapeutic strategy for CIPS. | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 書誌情報 |
en : Molecular pain 巻 22, p. 17448069251414260, 発行日 2026-01-03 |
|||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | SAGE Publications | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| ISSN | ||||||||||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||||||||||
| 収録物識別子 | 17448069 | |||||||||||||||||||||||
| DOI | ||||||||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1177/17448069251414260 | |||||||||||||||||||||||
| 権利 | ||||||||||||||||||||||||
| 権利情報 | © The Author(s) 2026. | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
