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Development of a Porcine Cell Line Stably Expressing Ephrin-B2 for Nipah Virus Research and Diagnostic Testing
http://hdl.handle.net/10458/0002002093
http://hdl.handle.net/10458/000200209331d574e8-537f-478c-a6e2-53ef63820e07
| 名前 / ファイル | ライセンス | アクション |
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2025-12-24 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Development of a Porcine Cell Line Stably Expressing Ephrin-B2 for Nipah Virus Research and Diagnostic Testing | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| キーワード | Nipah virus | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| キーワード | PK‐15 | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| キーワード | ephrin‐B2 | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| キーワード | interferon | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| キーワード | isolation and propagation | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ | journal article | |||||||||||||
| アクセス権 | ||||||||||||||
| アクセス権 | open access | |||||||||||||
| 著者 |
Zhang, Hui
× Zhang, Hui
× 齊藤, 暁
WEKO
33890
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Nipah virus (NiV) is a highly pathogenic zoonotic virus transmitted from bats to humans through pigs as a crucial intermediate host. NiV outbreaks pose significant public health and economic threats, especially for pig farmers. Although the World Organization for Animal Health recommends African green monkey-derived Vero cells for NiV isolation, pig-derived cell lines could represent an optimal platform for propagating pig-origin NiV, as signs and symptoms of NiV infection differ among different hosts. In this study, we generated and evaluated pig-derived PK-15 cells stably expressing pig-derived ephrin-B2 (PK-15/Ephrin-B2 cells), the primary receptor for NiV. NiV pseudovirus infectivity was increased by > 1000-fold in PK-15/Ephrin-B2 cells compared with that in wild-type PK-15 cells, whereas virus susceptibility was higher in PK-15/Ephrin-B2 cells than in Vero cells (> 30-fold). Furthermore, Stat2-knockout PK-15/Ephrin-B2 cells exhibited stable viral infectivity in the presence of type I interferon, making it particularly suitable for clinical sample testing. Moreover, PK-15/Ephrin-B2 cells proved useful for neutralization tests using anti-NiV hyperimmune ascitic fluid. Therefore, PK-15 cells expressing pig ephrin-B2 could represent an efficient tool for virus isolation, vaccine development, and virological studies of NiV and related henipaviruses. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : Microbiology and immunology 発行日 2025-11-17 |
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| 出版者 | Wiley | |||||||||||||
| 言語 | en | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 13480421 | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1111/1348-0421.70022 | |||||||||||||
| 権利 | ||||||||||||||
| 権利情報 | © 2025 The Author(s). | |||||||||||||
| 言語 | en | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
