| 著者 |
Dong, Thi-Thu-Trang
| en |
Dong, Thi-Thu-Trang（Personal）
Senior Official Institute, 108 Military Central Hospital
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Honda, Hiroyuki
| en |
Honda, Hiroyuki（Personal）
National Hospital Organization, Omuta National Hospital
|
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Akagi, Akio
Iwasaki, Yasushi
Kishida, Hitaru
Tsukamoto, Tadashi
| en |
Tsukamoto, Tadashi（Personal）
Research on Policy Planning and Evaluation for Rare and Intractable Diseases
|
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Kasuga, Kensaku
| en |
Kasuga, Kensaku（Personal）
Japan Prion Disease Surveillance Committee (J-PrD-SC)
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Takashima, Hirotsugu
| en |
Takashima, Hirotsugu（Personal）
National Epilepsy Center, NHO Shizuoka Institute of Epilepsy and Neurological Disorders (NEC)
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Terada, Tatsuhiro
| en |
Terada, Tatsuhiro（Personal）
National Epilepsy Center, NHO Shizuoka Institute of Epilepsy and Neurological Disorders (NEC)
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Ikenaka, Kensuke
| en |
Ikenaka, Kensuke（Personal）
Japan Prion Disease Surveillance Committee (J-PrD-SC)
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Ikeuchi, Takeshi
森, 剛志
WEKO
35474
e-Rad_Researcher
40426565
| ja |
森, 剛志
宮崎大学
|
| ja-Kana |
モリ, ツヨシ
|
| en |
Mori, Tsuyoshi
University of Miyazaki
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Mochizuki, Hideki
| en |
Mochizuki, Hideki（Personal）
Japan Prion Disease Surveillance Committee (J-PrD-SC)
|
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Ono, Kenjiro
Takiyama, Yoshihisa
Hamaguchi, Tsuyoshi
| en |
Hamaguchi, Tsuyoshi（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases
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Murota, Hiroyuki
Sanjo, Nobuo
| en |
Sanjo, Nobuo（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases
|
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Fujimoto, Takeshi
Kitayama, Michio
Fujita, Koji
Yukitake, Motohiro
| en |
Yukitake, Motohiro（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
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Fujioka, Shinsuke
| en |
Fujioka, Shinsuke（Personal）
International University of Health and Welfare
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Nishida, Noriyuki
Tsuboi, Yoshio
| en |
Tsuboi, Yoshio（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases
|
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Kitamoto, Tetsuyuki
| en |
Kitamoto, Tetsuyuki（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases
|
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Takao, Masaki
| en |
Takao, Masaki（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases
|
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Yamada, Masahito
| en |
Yamada, Masahito（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases
|
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Mizusawa, Hidehiro
| en |
Mizusawa, Hidehiro（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases
|
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Satoh, Katsuya
| en |
Satoh, Katsuya（Personal）
Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases
|
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内容記述 |
Invasive tests like cerebrospinal fluid (CSF) examination are highly effective for diagnosing human prion disease (HPD). Real-time quaking-induced conversion (RT-QuIC) CSF assay demonstrates 80-90% sensitivity for HPD diagnosis. To establish a minimally invasive diagnostic approach, we evaluated RT-QuIC testing on hair root and scalp samples. We collected scalp samples from 22 HPD and 5 non-HPD patients during pathological examinations and analyzed them using RT-QuIC assay and neuropathological methods. In our prospective study, hair root and CSF samples from 300 patients were tested using RT-QuIC assay along with other biomarkers, including 14-3-3 protein, total tau protein, RT-QuIC CSF assay, and MRI findings. All 22 HPD patients demonstrated positive prion seeding activity in scalp and hair root RT-QuIC assays. Neuropathological examination in one HPD patient revealed abnormal prion protein in scalp tissue. Among 177 HPD patients diagnosed by Japan Prion Surveillance Committee and 123 non-HPD patients, RT-QuIC assay of hair roots demonstrated sensitivity and specificity of 45.8% and 100%, respectively. Sensitivities of 14-3-3 protein, total tau protein, RT-QuIC CSF assay, and MRI findings were 83.1%, 86.4%, 74.6%, and 100%, respectively, with specificities of 65.0%, 65.0%, 100%, and 56.1%, respectively. RT-QuIC assays could be developed into novel diagnostic methods for neurodegenerative diseases. |
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