| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-12-18 |
| タイトル |
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|
タイトル |
Development of multiplex reverse-transcription digital PCR assay for co-detection of bovine leukemia virus and bovine viral diarrhea virus in cattle |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Bovine leukemia virus |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Bovine viral diarrhea virus |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Co-detection |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Multiplex RT-digital PCR assay |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Ullah, Shakir
Notsu, Kosuke
齊藤, 暁
WEKO
33890
e-Rad_Researcher
30621792
| ja |
齊藤, 暁
宮崎大学
|
| ja-Kana |
サイトウ, アカツキ
|
| en |
Saito, Akatsuki
University of Miyazaki
|
Search repository
岡林, 環樹
WEKO
33891
e-Rad_Researcher
10359995
| ja |
岡林, 環樹
宮崎大学
|
| ja-Kana |
オカバヤシ, タマキ
|
| en |
Okabayashi, Tamaki
University of Miyazaki
|
Search repository
目堅, 博久
WEKO
24382
e-Rad_Researcher
90633264
| ja |
目堅, 博久
宮崎大学
|
| ja-Kana |
メカタ, ヒロヒサ
|
| en |
Mekata, Hirohisa
University of Miyazaki
|
Search repository
Shiokawa, Mai
| en |
Shiokawa, Mai（Personal）
Nippon Veterinary and Life Science University
|
Search repository
Aoki, Hiroshi
| en |
Aoki, Hiroshi（Personal）
Nippon Veterinary and Life Science University
|
Search repository
関口, 敏
WEKO
24383
e-Rad_Researcher
10462780
| ja |
関口, 敏
宮崎大学
|
| ja-Kana |
セキグチ, サトシ
|
| en |
Sekiguchi, Satoshi
University of Miyazaki
|
Search repository
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Bovine leukemia virus (BLV) and bovine viral diarrhea virus (BVDV) are important transboundary pathogens that cause substantial economic losses in the cattle industry globally. Their early detection and control are critical for preventing disease transmission and minimizing their impact on livestock health and productivity. Therefore, establishing a sensitive and robust diagnostic method capable of simultaneously detecting BLV and BVDV is vital for implementing timely control measures. Here, we developed a multiplex RT-dPCR assay to detect BLV and BVDV in a single-tube reaction using nucleic acid extracted from whole blood of infected cattle. The multiplex RT-dPCR assay successfully detected both BLV and BVDV with high specificity, exhibiting no cross-reactivity with other bovine viruses including Akabane virus, bovine coronavirus, bovine parainfluenza virus 3, bovine respiratory syncytial virus, bovine herpesvirus 1, and bovine immunodeficiency virus. The assay demonstrated the ability to detect BVDV-1 and BVDV-2 at minimum titers of 10² and 10 ³ TCID₅₀/mL, respectively. For BLV, the multiplex RT-dPCR assay exhibited a detection limit as low as 18.7 viral copies. Our findings represent a significant advance in the detection of BLV and BVDV from extracted RNA and cDNA, highlighting the potential of assays for achieving improved diagnostic accuracy and early disease intervention. |
|
言語 |
en |
| 書誌情報 |
en : Journal of virological methods
巻 340,
p. 115298,
発行日 2026-02
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| 出版者 |
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出版者 |
Elsevier BV |
|
言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
18790984 |
| DOI |
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関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.jviromet.2025.115298 |
| 権利 |
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|
権利情報 |
© 2025 The Author(s). Published by Elsevier B.V. |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
Fulltext (2.4 MB)
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