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Magnetic Nanoparticle Capture and Quantum Dot Labeling for Rapid and Quantitative Detection of Methicillin-Resistant
http://hdl.handle.net/10458/0002001814
http://hdl.handle.net/10458/0002001814ce8c8f93-8703-4311-af1d-70500d662dde
| 名前 / ファイル | ライセンス | アクション |
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2025-10-07 | |||||||||||||||||||||
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| タイトル | Magnetic Nanoparticle Capture and Quantum Dot Labeling for Rapid and Quantitative Detection of Methicillin-Resistant | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||
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| 資源タイプ | journal article | |||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||
| 著者 |
Satheesan, Nijil
× Satheesan, Nijil
× マドゥエスタ, ハリーシャクマール
WEKO
7810
× 境, 健太郎
WEKO
12634
× Kini, Sudarshan
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| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | The increasing incidence of drug-resistant bacterial strains, such as methicillin-resistant (MRSA), poses a significant threat to public health owing to their high morbidity and mortality rates. Conventional methods for MRSA detection are expensive and time-consuming, necessitating advanced technological solutions. This study aimed to develop a direct detection strategy for MRSA by using magnetic nanoparticles (MNPs) as capture probes and quantum dots (QDs) as fluorescent detection probes. MNP capture probes were prepared by the coprecipitation of Fe3O4 NPs, followed by co-condensation to obtain amine-functionalized silica coatings on Fe3O4 NPs (amine-Si@MNPs). Carboxyl modification of amine-Si@MNPs was achieved via the hydrolytic cleavage of succinic anhydride. QDs were prepared using a one-pot chemical reduction method for CdTe core, followed by inorganic epitaxial growth of ZnS shell (CdTe/ZnS QDs). The size, morphology, surface functionalization, and magnetic and optical properties of the nanoprobes were characterized using several techniques. Both the nanoprobes were conjugated to protein A using carbodiimide chemistry. QD-protein A was conjugated to an anti-PBP2a antibody that specifically targets MRSA. In contrast, MNP-protein A was conjugated with human IgG to target outer membrane protein A of MRSA. MRSA detection was achieved by mixing the sample with the QD detection probe, followed by MNP capture probe, magnetic separation, and fluorescence measurements. Detection was achieved within ∼25 min, with a detection limit of 5 colony-forming units (CFU)/mL. The selectivity of this method was investigated by using mixed cultures of various bacterial isolates. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 書誌情報 |
en : ACS omega 巻 10, 号 29, p. 32189-32201, 発行日 2025-07-18 |
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| 出版者 | American Chemical Society | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||||
| 収録物識別子 | 24701343 | |||||||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1021/acsomega.5c03973 | |||||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||||
