| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-08-27 |
| タイトル |
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タイトル |
Performance evaluation of an improved RAISING method for clonality analysis of bovine leukemia virus-infected cells: a collaborative study in Japan |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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キーワード |
bovine leukemia virus |
| キーワード |
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言語 |
en |
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キーワード |
clonality analysis |
| キーワード |
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言語 |
en |
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キーワード |
enzootic bovine leukosis |
| キーワード |
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言語 |
en |
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キーワード |
multicenter interlaboratory validation |
| キーワード |
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言語 |
en |
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キーワード |
RAISING |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Okagawa, Tomohiro
Nojiri, Naomi
Yoshida-Furihata, Hazuka
| en |
Yoshida-Furihata, Hazuka
National Institute of Infectious Diseases
|
Search repository
Nao, Naganori
Tominaga, Misono
Kohara, Junko
Gondaira, Satoshi
Higuchi, Hidetoshi
Takeda, Yohei
| en |
Takeda, Yohei
Obihiro University of Agriculture and Veterinary Medicine
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Search repository
Ogawa, Haruko
| en |
Ogawa, Haruko
Obihiro University of Agriculture and Veterinary Medicine
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Search repository
Yamada, Shinji
Murakami, Kenji
Suzuki, Yasunori
Takai, Shinji
Maezawa, Masaki
Inokuma, Hisashi
Shimizu, Kaori
Inoshima, Yasuo
Usui, Tatsufumi
Tagawa, Michihito
Yamamoto, Mari
目堅, 博久
WEKO
24382
e-Rad_Researcher
90633264
| ja |
目堅, 博久
宮崎大学
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| ja-Kana |
メカタ, ヒロヒサ
|
| en |
Mekata, Hirohisa
University of Miyazaki
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Search repository
Esaki, Mana
Ozawa, Makoto
Matsudaira, Takahiro
Maekawa, Naoya
Murata, Shiro
Ohashi, Kazuhiko
Saito, Masumichi
Konnai, Satoru
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Bovine leukemia virus (BLV), a retrovirus that is widespread worldwide, causes enzootic bovine leukosis (EBL), a B-cell leukemia/lymphoma with a poor prognosis that ultimately results in death. In Japan, the number of cattle infected with this virus is increasing, and it is estimated more than 35% of cattle are currently infected. Since no vaccines or treatments against BLV infection are currently available, it is important to establish a method of early diagnosis for EBL to reduce economic losses caused by the disposal of EBL cattle in Japan, where a large number of expensive beef cattle are raised. We previously developed Rapid Amplification of the Integration Site without Interference by Genomic DNA Contamination (RAISING), a cost-effective, rapid, and sensitive method for the clonality analysis of BLV-infected cells. Despite its usefulness for the early diagnosis of EBL, RAISING had drawbacks preventing its practical application. Here, we report the development of an improved method, RAISING ver.2, and its performance. Compared to BLV clonality analysis using the previous method, RAISING ver.2 was found to maintain high accuracy and reproducibility despite its simplification. Moreover, its performance was also validated in a multicenter validation study. Taken together, our results strongly suggest that RAISING ver.2 can be fully utilized in clinical practice. Successful commercialization of a RAISING test kit could overcome the concerns of livestock farmers suffering from EBL, thereby promoting a stable supply of Japanese beef, both domestically and internationally. |
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言語 |
en |
| 書誌情報 |
en : Journal of Veterinary Medical Science
巻 87,
号 5,
p. 551-558,
発行日 2025
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| 出版者 |
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出版者 |
Japanese Society of Veterinary Science |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
13477439 |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
09167250 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1292/jvms.25-0031 |
| 権利 |
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権利情報 |
© 2025 by the Japanese Society of Veterinary Science |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
Fulltext (3.3 MB)
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