| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-08-27 |
| タイトル |
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タイトル |
Structural insights into lab-coevolved RNA-RBP pairs and applications of synthetic riboswitches in cell-free system |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
福永, 圭佑
WEKO
35646
| ja |
福永, 圭佑
宮崎大学
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| ja-Kana |
フクナガ, ケイスケ
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| en |
Fukunaga, Keisuke
University of Miyazaki
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Search repository
Teramoto, Takamasa
Nakashima, Momoka
Ohtani, Toshitaka
Katsuki, Riku
Matsuura, Tomoaki
Yokobayashi, Yohei
| en |
Yokobayashi, Yohei
Okinawa Institute of Science and Technology Graduate University
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Search repository
Kakuta, Yoshimitsu
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
CS1-LS4 and CS2-LS12 are ultra-high affinity and orthogonal RNA-protein pairs that were identified by PD-SELEX (Phage Display coupled with Systematic Evolution of Ligands by EXponential enrichment). To investigate the molecular basis of the lab-coevolved RNA-RBP pairs, we determined the structures of the CS1-LS4 and CS2-LS12 complexes and the LS12 homodimer in an RNA-free state by X-ray crystallography. The structural analyses revealed that the lab-coevolved RNA-RBPs have acquired unique molecular recognition mechanisms, whereas the overall structures of the RNP complexes were similar to the typical kink-turn RNA-L7Ae complex. The orthogonal RNA-RBP pairs were applied to construct high-performance cell-free riboswitches that regulate translation in response to LS4 or LS12. In addition, by using the orthogonal protein-responsive switches, we generated an AND logic gate that outputs staphylococcal γ-hemolysin in cell-free system and carried out hemolysis assay and calcein leakage assay using rabbit red blood cells and artificial cells, respectively. |
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言語 |
en |
| 書誌情報 |
en : Nucleic Acids Research
巻 53,
号 6,
p. gkaf212,
発行日 2025-05-22
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| 出版者 |
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出版者 |
Oxford University Press |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
13624962 |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
03051048 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/nar/gkaf212 |
| 権利 |
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権利情報 |
© The Author(s) 2025. |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
Fulltext (1.7 MB)
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