WEKO3
アイテム
Direct inhibition of C-type lysozyme in Japanese flounder by lysozyme inhibitor Ivy derived from Edwardsiella piscicida
http://hdl.handle.net/10458/0002001414
http://hdl.handle.net/10458/0002001414c5619f0f-d33a-4750-8666-e494e5445394
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Manuscript (558 KB)
Download is available from 2026/9/30.
|
.png)
|
|
|
Figures (550 KB)
Download is available from 2026/9/30.
|
|
|
|
Supplementary data (907 KB)
Download is available from 2026/9/30.
|
|
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-06-23 | |||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||
| タイトル | Direct inhibition of C-type lysozyme in Japanese flounder by lysozyme inhibitor Ivy derived from Edwardsiella piscicida | |||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| キーワード | C-type lysozyme | |||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| キーワード | Edwardsiella piscicida | |||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| キーワード | Edwardsiellosis | |||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| キーワード | Inhibitor of vertebrate lysozyme | |||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| キーワード | Japanese flounder Paralichthys olivaceus | |||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||
| 著者 |
Nishihara, Aki
× Nishihara, Aki
× Sumiyoshi, Takechiyo
× Washim, Mizanur Rahman
× Yasumoto, Shinya
× Kondo, Masakazu
× 河野, 智哉
WEKO
3674
× 引間, 順一
WEKO
34515
|
|||||||||||||||||||||||||||
| 抄録 | ||||||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||||||
| 内容記述 | Edwardsiella piscicida (Enterobacteriaceae) is an important fish pathogen that causes edwardsiellosis in many species, including Japanese flounder (Paralichthys olivaceus), leading to economic losses in aquaculture. We investigated the direct inhibitory activity of IvyEp (Inhibitors of vertebrate lysozyme derived from E. piscicida) against host C-type lysozyme, and its role in host immune evasion. The results of functional assays confirmed that IvyEp significantly inhibited the activity of hen egg white lysozyme (HEWL), and kinetic analysis demonstrated that IvyEp acts as a competitive inhibitor. The ivy-deleted strain of E. piscicida exhibited reduced virulence in Japanese medaka (Oryzias latipes) and flounder infection models, highlighting its role in immune evasion and pathogenicity. IvyEp significantly suppressed lysozyme-like activity in the serum of Japanese flounder, yellowtail (Seriola quinqueradiata), and Nile tilapia (Oreochromis niloticus), suggesting broad-spectrum inhibition of C-type lysozymes across these species. Further assays confirmed that IvyEp significantly inhibited the lytic activity of purified flounder C-type lysozyme (rLyzC), supporting the hypothesis that IvyEp directly interferes with host lysozyme activity. Structural analysis predicted that IvyEp blocks the active site of rLyzC through electrostatic interactions, preventing substrate binding, and thus neutralizing rLyzC function. Co-immunoprecipitation revealed that IvyEp directly binds to HEWL and rLyzC. These findings provide new insights into the potential role of IvyEp in protecting E. piscicida from host immune responses and suggest its probable function as a universal C-type lysozyme inhibitor across different fish species. | |||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| bibliographic_information |
en : Aquaculture 巻 608, p. 742707, 発行日 2025-09-30 |
|||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||
| 出版者 | Elsevier BV | |||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| ISSN | ||||||||||||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||||||||||||
| 収録物識別子 | 00448486 | |||||||||||||||||||||||||||
| item_10001_relation_14 | ||||||||||||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.aquaculture.2025.742707 | |||||||||||||||||||||||||||
| 権利 | ||||||||||||||||||||||||||||
| 権利情報 | © 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies. | |||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||||||||
