| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-25 |
| タイトル |
|
|
タイトル |
Development of a Multi-Locus Real-Time PCR with a High-Resolution Melting Assay to Differentiate Wild-Type, Asian Recombinant, and Vaccine Strains of Lumpy Skin Disease Virus |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
キーワード |
DIVA |
| キーワード |
|
|
言語 |
en |
|
キーワード |
high-resolution melting analysis |
| キーワード |
|
|
言語 |
en |
|
キーワード |
lumpy skin disease virus |
| キーワード |
|
|
言語 |
en |
|
キーワード |
multi-locus |
| キーワード |
|
|
言語 |
en |
|
キーワード |
real-time PCR |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
| 著者 |
Bhakha, Kultyarat
Matsui, Yuto
Buakhao, Natchaya
Wanganurakkul, Saruda
| en |
Wanganurakkul, Saruda
Veterinary Research and Development Center (Eastern Region)
|
Search repository
Deemagarn, Taweewat
| en |
Deemagarn, Taweewat
Thailand National Institute of Animal Health
|
Search repository
Oba, Mami
Takemae, Hitoshi
Mizutani, Tetsuya
| en |
Mizutani, Tetsuya
Tokyo University of Agriculture and Technology
|
Search repository
三澤, 尚明
WEKO
1864
e-Rad_Researcher
20229678
| ja |
三澤, 尚明
宮崎大学
|
| ja-Kana |
ミサワ, ナオアキ
|
| en |
Misawa, Naoaki
University of Miyazaki
|
Search repository
Chintapitaksakul, Lerdchai
| en |
Chintapitaksakul, Lerdchai
Thailand National Institute of Animal Health
|
Search repository
山田, 健太郎
WEKO
34098
e-Rad_Researcher
70458280
| ja |
山田, 健太郎
宮崎大学
|
| ja-Kana |
ヤマダ, ケンタロウ
|
| en |
Yamada, Kentaro
University of Miyazaki
|
Search repository
Suwankitwat, Nutthakarn
| en |
Suwankitwat, Nutthakarn
Thailand National Institute of Animal Health
|
Search repository
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Lumpy skin disease virus (LSDV) affects cattle and causes significant economic damage. The live vaccine derived from an attenuated strain is effective but is associated with mild disease and skin lesions in some vaccinated cattle. Moreover, recombinant LSDV strains, particularly one with wild-type field and vaccine strains, have recently emerged and spread throughout Asian countries. A cost-effective LSDV typing method is required. We developed a multi-locus real-time PCR with a high-resolution melting (HRM) assay to differentiate between the wild-type, vaccine, and dominant Asian recombinant strains. Based on a multiple alignment analysis, we selected three target genes for the HRM assay, ORF095, ORF126, and ORF145, in which there are insertions/deletions and nucleotide substitutions between wild-type and vaccine strains, and designed primer sets for the assay. Using the synthetic DNA encoding these genes for the two strains, it was shown that the PCR amplicons intercalated with a saturating fluorescent dye could clearly differentiate between wild-type and vaccine strains in the HRM analysis for all three target genes. Further, using clinical samples, our method was able to identify recombinant strains harboring the wild-type ORF095 and ORF145 and the vaccine strain ORF126 genes. Thus, our HRM assay may provide rapid LSDV typing. |
|
言語 |
en |
| bibliographic_information |
en : Veterinary Sciences
巻 12,
号 3,
p. 213,
発行日 2025-03-01
|
| 出版者 |
|
|
出版者 |
MDPI AG |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
23067381 |
| item_10001_relation_14 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/vetsci12030213 |
| 権利 |
|
|
権利情報 |
© 2025 by the authors. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
fulltext (2.1 MB)
.png)
