| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-25 |
| タイトル |
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タイトル |
Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Kokubo-Tanaka, Mio
Kildemoes, Anna Overgaard
Chadeka, Evans Asena
Cheruiyot, Benard Ngetich
Moriyasu, Taeko
Sassa, Miho
Nakamura, Risa
Kikuchi, Mihoko
Fujii, Yoshito
de Dood, Claudia J.
Corstjens, Paul L.A.M.
Kaneko, Satoshi
丸山, 治彦
WEKO
3231
e-Rad_Researcher
90229625
| ja |
丸山, 治彦
宮崎大学
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| ja-Kana |
マルヤマ, ハルヒコ
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| en |
Maruyama, Haruhiko
University of Miyazaki
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Search repository
Njenga, Sammy M.
de Vrueh, Remco
Hokke, Cornelis Hendrik
Hamano, Shinjiro
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Background
Schistosoma haematobium is the causative pathogen for urogenital schistosomiasis. To achieve progress towards schistosomiasis elimination, there is a critical need for developing highly sensitive and specific tools to monitor transmission in near-elimination settings. Although antibody detection is a promising approach, it is usually unable to discriminate active infections from past ones. Moreover, crude antigens such as soluble egg antigen (SEA) show cross-reactivity with other parasitic infections, and it is difficult to formulate the standard preparations. To resolve these issues, the performances of recombinant antigens have been evaluated. The antibody responses against recombinant S. haematobium serine-protease inhibitor (ShSerpin) and RP26 were previously shown to reflect active schistosome infection in humans. Furthermore, antibody detection using multiple recombinant antigens has been reported to improve the accuracy of antibody-based assays compared to single-target assays. Therefore, we examined the performances of ShSerpin, RP26 and the mixture of these antigens for detecting S. haematobium low-intensity infection and assessed the potential for transmission monitoring.
Methodology/principal findings
We collected urine and plasma samples from school-aged children in Kwale, Kenya and evaluated S. haematobium prevalence by number of eggs in urine and worm-derived circulating anodic antigen (CAA) in plasma. Among 269 pupils, 50.2% were CAA-positive by the lateral flow test utilizing up-converting phosphor particles (UCP-LF CAA), while only 14.1% were egg-positive. IgG levels to S. haematobium SEA (ShSEA), ShSerpin, RP26, and the mixture of ShSerpin and RP26 were measured by ELISA. The mixture of ShSerpin and RP26 showed the highest sensitivity, 88.7%(125/141)among the four antigens in considering indecisive UCP-LF CAA results as negative.
Conclusion/significance
IgG detection against the ShSerpin-RP26 mixture demonstrated better sensitivity for detection of active S. haematobium infection. This recombinant antigen mixture is simpler to produce with higher reproducibility and can potentially replace ShSEA in monitoring transmission under near-elimination settings. |
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言語 |
en |
| bibliographic_information |
en : PLoS Neglected Tropical Diseases
巻 19,
号 1,
発行日 2025-01-01
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| 出版者 |
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出版者 |
Public Library of Science (PLoS) |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
19352727 |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
19352735 |
| item_10001_relation_14 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pntd.0012813 |
| 出版タイプ |
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出版タイプ |
VoR |
fulltext (1.8 MB)
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