| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-25 |
| タイトル |
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タイトル |
Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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キーワード |
Antibiotics |
| キーワード |
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言語 |
en |
|
キーワード |
antimicrobial agents |
| キーワード |
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言語 |
en |
|
キーワード |
bovine respiratory disease |
| キーワード |
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言語 |
en |
|
キーワード |
extracellular matrix |
| キーワード |
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|
言語 |
en |
|
キーワード |
trachea |
| 資源タイプ |
|
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Nishi, Koji
Gondaira, Satoshi
Hirano, Yuki
Ohashi, Masahide
Sato, Ayano
Matsuda, Kazuya
Iwasaki, Tomohito
Kanda, Takuya
上村, 涼子
WEKO
33773
e-Rad_Researcher
90529190
| ja |
上村, 涼子
宮崎大学
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| ja-Kana |
ウエムラ, リョウコ
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| en |
Uemura, Ryoko
University of Miyazaki
|
Search repository
Higuchi, Hidetoshi
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions. A recent study has shown that polymicrobial interactions between species are an important factor in biofilm formation, yet the precise mechanism of biofilm formation in M. bovis remains unknown. By assuming multiple pathogen infections in the bovine respiratory disease complex (BRDC), this study examined the characterisation of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Autopsies were performed on four Holstein calves (two chronic Mycoplasma pneumonia calves and two control calves). Bacterium-like aggregation structures (> 10 μm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released an extracellular matrix to connect with neighbouring bacteria and form a mature biofilm on the plate. Biofilm formation in the co-culture of M. bovis and T. pyogenes (strain T1: 1 × 105 and 1 × 106 CFU/well) significantly increased (p < 0.05 and p < 0.01; 64.1% and 64.8% increase) compared to that in a single culture of these bacteria. Furthermore, some large aggregates (> 40 μm), composed of M. bovis and T. pyogenes, were observed. The morphological characteristics of this biofilm were similar to those observed in vivo compared to a single culture. In conclusion, the polymicrobial interaction between M. bovis and T. pyogenes induces biofilm formation, which is associated with increased resistance to antimicrobial agents, and this exacerbates the progression of chronic Mycoplasma pneumonia. |
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言語 |
en |
| bibliographic_information |
en : Veterinary research
巻 56,
号 1,
p. 22,
発行日 2025-01-30
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| 出版者 |
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出版者 |
Springer Science and Business Media LLC |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
12979716 |
| item_10001_relation_14 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1186/s13567-025-01468-1 |
| 権利 |
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権利情報 |
© The Author(s) 2025 |
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言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
fulltext (11 MB)
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