| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-08 |
| タイトル |
|
|
タイトル |
ER-mitochondria contacts mediate lipid radical transfer via RMDN3/PTPIP51 phosphorylation to reduce mitochondrial oxidative stress |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
| 著者 |
Shiiba, Isshin
Ito, Naoki
Oshio, Hijiri
Ishikawa, Yuto
Nagao, Takahiro
Shimura, Hiroki
Oh, Kyu-Wan
Takasaki, Eiki
Yamaguchi, Fuya
Konagaya, Ryoan
門脇, 寿枝
WEKO
34447
e-Rad_Researcher
40568200
| ja |
門脇, 寿枝
宮崎大学
|
| ja-Kana |
カドワキ, ヒサエ
|
| en |
Kadowaki, Hisae
University of Miyazaki
|
Search repository
西頭, 英起
WEKO
34317
e-Rad_Researcher
00332627
| ja |
西頭, 英起
宮崎大学
|
| ja-Kana |
ニシトウ, ヒデキ
|
| en |
Nishitoh, Hideki
University of Miyazaki
|
Search repository
Tanzawa, Takehito
Nagashima, Shun
Sugiura, Ayumu
Fujikawa, Yuuta
Umezawa, Keitaro
Tamura, Yasushi
Il Lee, Byung
Hirabayashi, Yusuke
Okazaki, Yasushi
Sawa, Tomohiro
Inatome, Ryoko
Yanagi, Shigeru
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The proximal domains of mitochondria and the endoplasmic reticulum (ER) are linked by tethering factors on each membrane, allowing the efficient transport of substances, including lipids and calcium, between them. However, little is known about the regulation and function of mitochondria-ER contacts (MERCs) dynamics under mitochondrial damage. In this study, we apply NanoBiT technology to develop the MERBiT system, which enables the measurement of reversible MERCs formation in living cells. Analysis using this system suggests that induction of mitochondrial ROS increases MERCs formation via RMDN3 (also known as PTPIP51)-VAPB tethering driven by RMDN3 phosphorylation. Disruption of this tethering caused lipid radical accumulation in mitochondria, leading to cell death. The lipid radical transfer activity of the TPR domain in RMDN3, as revealed by an in vitro liposome assay, suggests that RMDN3 transfers lipid radicals from mitochondria to the ER. Our findings suggest a potential role for MERCs in cell survival strategy by facilitating the removal of mitochondrial lipid radicals under mitochondrial damage. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Citation: Isshin Shiiba, Naoki Ito, Hijiri Oshio, Yuto Ishikawa, Takahiro Nagao, Hiroki Shimura, Kyu-Wan Oh, Eiki Takasaki, Fuya Yamaguchi, Ryoan Konagaya, Hisae Kadowaki, Hideki Nishitoh, Takehito Tanzawa, Shun Nagashima, Ayumu Sugiura, Yuuta Fujikawa, Keitaro Umezawa, Yasushi Tamura, Byung Il Lee, Yusuke Hirabayashi, Yasushi Okazaki, Tomohiro Sawa, Ryoko Inatome, Shigeru Yanagi, ER-mitochondria contacts mediate lipid radical transfer via RMDN3/PTPIP51 phosphorylation to reduce mitochondrial oxidative stress, Nature Communications, 16(1), 2025-02-10, https://doi.org/10.1038/s41467-025-56666-4 |
|
言語 |
en |
| bibliographic_information |
en : Nature Communications
巻 16,
号 1,
発行日 2025-02-10
|
| 出版者 |
|
|
出版者 |
Springer Science and Business Media LLC |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2041-1723 |
| item_10001_relation_14 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1038/s41467-025-56666-4 |
| 権利 |
|
|
権利情報 |
© The Author(s) 2025 |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
Fulltext (8.2 MB)
.png)
