| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-07 |
| タイトル |
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タイトル |
Investigating BoLA Class II DRB3*009:02 carrying cattle in Japan |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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キーワード |
BoLA-DRB3×009:02 |
| キーワード |
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言語 |
en |
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キーワード |
Bovine leukemia virus |
| キーワード |
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言語 |
en |
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キーワード |
PCR-RFLP |
| キーワード |
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言語 |
en |
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キーワード |
Productivity |
| キーワード |
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言語 |
en |
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キーワード |
Seroprevalence |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Fujimori, Sho
Ando, Tatsuya
関口, 敏
WEKO
24383
e-Rad_Researcher
10462780
| ja |
関口, 敏
宮崎大学
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| ja-Kana |
セキグチ, サトシ
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| en |
Sekiguchi, Satoshi
University of Miyazaki
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Search repository
野津, 昂亮
Ishida, Shogo
Daidoji, Tomo
Hagiwara, Katsuro
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Enzootic bovine leukosis (EBL) is a malignant lymphoma of cattle that is mainly caused by bovine leukemia virus (BLV) infection. In this study, PCR-RFLP was used to investigate the frequency of the DRB3*009:02 allele in several farms with different herd management practices in Japan. A total of 742 Holsteins (384) and Japanese Blacks (230) were used as the sample size for the study, which was larger than the number of cattle in the study area with a confidence level of 95 % and a margin of error of 8. PBMCs isolated from whole blood from clinically healthy cattle were used for examination. The presence of BLV provirus infection was determined by qPCR targeting the env region. BLV antibodies were detected using a commercial ELISA kit. The results showed that 35 cattle were heterozygous for DRB3*009:02. The frequency on each farm varied between farms, and PCR analysis showed that the prevalence of BLV also varied between farms. The incidence rate (4.7 %) was lower than in previous studies. The BLV seroprevalence (14.4 %) in this study was lower than the BLV infection rate (35 %) in the study. Holstein dairy cows had low levels of BL resistance genes, confirming the spread of the virus within the farm's herd. Cattle on farms with low BLV-positive rates had a resistance gene-carrying rate of 16.9 %, meanwhile, those who did not carry the resistance gene had a rate of 91.4 %. BLV provirus levels vary between farmers, with herds carrying low BL-resistance genes tending to have higher levels of BLV provirus. In light of the current BLV epidemic, herd composition reform, along with aggressive breeding of BL-resistant sires, is a required component to increase the herd of BL-resistant sires and improve livestock productivity. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Citation: Sho Fujimori, Tatsuya Ando, Satoshi Sekiguchi, Kosuke Notsu, Shogo Ishida, Tomo Daidoji, Katsuro Hagiwara, Investigating BoLA Class II DRB3*009:02 carrying cattle in Japan, Veterinary and Animal Science, 27, 100425-100425, 2025-03, https://doi.org/10.1016/j.vas.2025.100425 |
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言語 |
en |
| bibliographic_information |
en : Veterinary and Animal Science
巻 27,
p. 100425,
発行日 2025-03
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| 出版者 |
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出版者 |
Elsevier BV |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
2451-943X |
| item_10001_relation_14 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.vas.2025.100425 |
| 権利 |
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権利情報 |
© 2025 The Author(s). |
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言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
Fulltext (647 KB)
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