| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-07 |
| タイトル |
|
|
タイトル |
Dynamics of macrophages and inflammatory cytokine concentrations in the cervix of late pregnant cows |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Cervical ripening |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Cows |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Interleukin-8 |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Macrophages |
| キーワード |
|
|
言語 |
en |
|
キーワード |
Mucus |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
| 著者 |
Kanemaru, Kazuyuki
北原, 豪
Hashiguchi, Takuto
Hemmi, Koichiro
小林, 郁雄
WEKO
5214
e-Rad_Researcher
20576293
| ja |
小林, 郁雄
宮崎大学
|
| ja-Kana |
コバヤシ, イクオ
|
| en |
Kobayashi, Ikuo
University of Miyazaki
|
Search repository
大澤, 健司
WEKO
21720
e-Rad_Researcher
90302059
| ja |
大澤, 健司
宮崎大学
|
| ja-Kana |
オオサワ, タケシ
|
| en |
Osawa, Takeshi
University of Miyazaki
|
Search repository
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Cervical ripening is associated with M1 macrophages and inflammatory cytokines in mice and rats, but its mechanism in cattle is unclear. In this study, to elucidate some of the mechanisms of normal cervical maturation in cows, we examined the expressions of M1 macrophages and mRNA of interleukin (IL)-1α, 1β, 6, 8, and 10, as well as TNFα in cervical tissue and the mRNA expressions and protein concentrations of these cytokines in cervical mucus from late pregnancy to parturition. Forty-one Japanese Black cows were sampled at 200, 230, and 260 d of gestation and then at 7 d intervals until parturition to obtain cervical mucus and tissue samples. The collected tissues were fluorescently immunostained with anti-Iba-1 antibodies, and the macrophage infiltration was checked. Cervical mucus was mixed with PBS, the concentrations of the above cytokines in the supernatant after centrifugation were measured, and the concentration per protein weight was used as the measurement. Total RNA was extracted from the cervical tissue and mucus, and the mRNA expression of various cytokines was analyzed using real-time PCR. In cervical tissue, macrophages stained with anti-Iba-1 antibody were observed from five to six weeks before calving until the week of calving. There was also a trend toward a concurrent increase in the mRNA expression of inflammatory cytokines, especially IL-6, three weeks before calving (P < 0.05). In cervical mucus, the concentrations of IL-1α, IL-1β, IL-8, and TNFα increased (P < 0.05) zero to three weeks before calving compared to 12–14 weeks before calving. In addition, IL-1α mRNA increased three weeks before and during the week of parturition, and IL-8 mRNA increased three weeks before and two weeks after (P < 0.05). These results suggest that cervical ripening in cows begins five to six weeks before calving when macrophages infiltrating cervical tissue may produce large amounts of IL-6, inflammatory cells infiltrate cervical mucus, and IL-1α, IL-1β, IL-8, and TNFα levels in the mucus increase toward calving. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Citation: Kazuyuki Kanemaru, Go Kitahara, Takuto Hashiguchi, Koichiro Hemmi, Ikuo Kobayashi, Takeshi Osawa, Dynamics of macrophages and inflammatory cytokine concentrations in the cervix of late pregnant cows, Theriogenology, 238, 117357-117357, 2025-05, https://doi.org/10.1016/j.theriogenology.2025.02.023 |
|
言語 |
en |
| bibliographic_information |
en : Theriogenology
巻 238,
p. 117357,
発行日 2025-05
|
| 出版者 |
|
|
出版者 |
Elsevier BV |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0093-691X |
| item_10001_relation_14 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.theriogenology.2025.02.023 |
| 権利 |
|
|
権利情報 |
© 2025 The Authors. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
Fulltext (2.6 MB)
.png)
