| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-02 |
| タイトル |
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タイトル |
Genetic diversity of dissolved free extracellular DNA compared to intracellular DNA in wastewater treatment plants |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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キーワード |
Antibiotic-resistance gene |
| キーワード |
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言語 |
en |
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キーワード |
Extracellular DNA |
| キーワード |
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言語 |
en |
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キーワード |
Intracellular DNA |
| キーワード |
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言語 |
en |
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キーワード |
Metagenomic analysis |
| キーワード |
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言語 |
en |
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キーワード |
Mobile genetic element |
| キーワード |
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言語 |
en |
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キーワード |
Wastewater treatment plant |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Tamai, Soichiro
Okuno, Miki
Ogura, Yoshitoshi
鈴木, 祥広
WEKO
5949
e-Rad_Researcher
90264366
| ja |
鈴木, 祥広
宮崎大学
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| ja-Kana |
スズキ, ヨシヒロ
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| en |
Suzuki, Yoshihiro
University of Miyazaki
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Search repository
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Dissolved free extracellular DNA (free-exDNA) coexists with intracellular DNA (inDNA) in aquatic environments. Free-exDNA can be taken up by bacteria through transformation, and wastewater treatment plants (WWTPs) are positioned as potential hot spots for genetic contamination. However, studies comparing the composition of free-exDNA and inDNA is limited. This study employed colloidal adsorption and foam concentration method to recover free-exDNA from different WWTP stages and compared its diversity with inDNA via metagenomic analysis. Free-exDNA concentrations were observed to increase after chlorination. Genetic analysis revealed a higher abundance of specific genes following chlorination, suggesting that free-exDNA in effluent originated from bacterial death in secondary treated water. This result indicates that free-exDNA, which increases due to chlorination, is subsequently released into the catchment. Additionally, several high-risk antibiotic-resistance genes (ARGs) were detected that colocalized with mobile genetic elements. These ARGs were expected to have a high potential for gene transfer via transformation, and the risk was highlighted. Overall, these findings deepen our understanding of horizontal gene transfer risks in WWTPs. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Citation: Soichiro Tamai, Miki Okuno, Yoshitoshi Ogura, Yoshihiro Suzuki, Genetic diversity of dissolved free extracellular DNA compared to intracellular DNA in wastewater treatment plants, Science of The Total Environment, 970, 178989-178989, 2025-03, https://doi.org/10.1016/j.scitotenv.2025.178989 |
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言語 |
en |
| bibliographic_information |
en : Science of The Total Environment
巻 970,
p. 178989,
発行日 2025-03-20
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| 出版者 |
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出版者 |
Elsevier BV |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0048-9697 |
| item_10001_relation_14 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.scitotenv.2025.178989 |
| 権利 |
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権利情報 |
© 2025 The Authors. |
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言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
Fulltext (7.9 MB)
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