WEKO3
アイテム
Loss of hepatocyte growth factor activator inhibitor type 1 upregulates MMP-9 expression and induces degradation of the epidermal basement membrane
http://hdl.handle.net/10458/0002001214
http://hdl.handle.net/10458/0002001214208d2be4-1d3e-46bb-8a04-ceba311193f4
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AcceptedManuscript (11.4 MB)
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This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections.
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||||
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| 公開日 | 2025-04-28 | |||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||
| タイトル | Loss of hepatocyte growth factor activator inhibitor type 1 upregulates MMP-9 expression and induces degradation of the epidermal basement membrane | |||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||
| キーワード | Basement membrane | |||||||||||||||||||||||||
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| 言語 | en | |||||||||||||||||||||||||
| キーワード | HAI-1 | |||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||
| キーワード | Keratinocyte | |||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||
| キーワード | MMP-9 | |||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||
| キーワード | NF-κB | |||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||
| 著者 |
Weiting, Liang
× Weiting, Liang
× 川口, 真紀子
WEKO
9881
× 福島, 剛
WEKO
9884
× 佐藤, 勇一郎
WEKO
9417
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| 抄録 | ||||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||||
| 内容記述 | Hepatocyte growth factor activator inhibitor type 1 (HAI-1), which is encoded by the SPINT1 gene, is a membrane-associated serine proteinase inhibitor abundantly expressed in epithelial tissues. We had previously demonstrated that HAI-1 is critical for placental development, epidermal keratinization, and maintenance of keratinocyte morphology by regulating cognate proteases, matriptase and prostasin. After performing ultrastructural analysis of Spint1-deleted skin tissues, our results showed that Spint1-deleted epidermis exhibited partially disrupted epidermal basement membrane structures. Matrix metalloproteinases-9 (MMP-9) expression levels were upregulated in Spint1-deleted primary cultured keratinocytes and SPINT1 knockout (KO) HaCaT cells. Furthermore, gelatin zymography of the conditioned medium showed increased MMP activities in keratinocytes with reduced HAI-1 expression. Treating SPINT1 KO HaCaT cells with dehydroxymethylepoxyquinomicin (DHMEQ), a small molecule inhibitor of NF-κB, abrogated the upregulation of MMP9 and the gelatinolytic activity associated with MMP9. These results suggest that HAI-1 may play a critical role in epidermal basement membrane integrity by regulating NF-κB activation-induced upregulation of MMP-9. | |||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||
| bibliographic_information |
en : Human Cell 巻 38, p. 36, 発行日 2024-12-28 |
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| 出版者 | ||||||||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||||||||
| 収録物識別子 | 1749-0774 | |||||||||||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s13577-024-01159-7 | |||||||||||||||||||||||||
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| 出版タイプ | AM | |||||||||||||||||||||||||
