| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-04-25 |
| タイトル |
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タイトル |
A cellulose-binding domain specific for native crystalline cellulose in lytic polysaccharide monooxygenase from the brown-rot fungus Gloeophyllum trabeum |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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キーワード |
Biodegradation |
| キーワード |
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言語 |
en |
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キーワード |
Brown-rot decay |
| キーワード |
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言語 |
en |
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キーワード |
Carbohydrate-binding protein |
| キーワード |
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言語 |
en |
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キーワード |
Cellulose |
| キーワード |
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言語 |
en |
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キーワード |
Fungi |
| キーワード |
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言語 |
en |
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キーワード |
Plant cell wall |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Kojima, Yuka
Sunagawa, Naoki
田川, 聡美
WEKO
35251
e-Rad_Researcher
20911952
| ja |
田川, 聡美
宮崎大学
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| ja-Kana |
タガワ, サトミ
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| en |
Tagawa, Satomi
University of Miyazaki
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Search repository
Hatano, Tomohiro
Aoki, Moeri
Kurei, Tatsuki
Horikawa, Yoshiki
Wada, Masahisa
Funada, Ryo
Igarashi, Kiyohiko
Yoshida, Makoto
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Cellulose-binding domains (CBDs) play a vital role in cellulose degradation by enzymes. Despite the strong ability of brown-rot fungi to degrade cellulose in wood, they have been considered to lack or have a low number of enzymes with CBD. Here, we report the C-terminal domain of a lytic polysaccharide monooxygenase from the brown-rot fungus Gloeophyllum trabeum (GtLPMO9A-2) functions as a CBD, classified as a new family of carbohydrate-binding module, CBM104. The amino acid sequence of GtCBM104 shows no similarity to any known CBDs. A BLAST search identified 84 homologous sequences at the C-terminus of some CAZymes, mainly LPMO9, in basidiomycetous genomes. Binding experiments revealed GtCBM104 binds selectively to native crystalline cellulose (cellulose I), but not to artificially modified crystalline or amorphous cellulose, while the typical fungal CBD (CBM1) bound to all cellulosic materials tested. The adsorption efficiency of GtCBM104 to cellulose I was >20-times higher than that of CBM1. Adsorption tests and microscopic observations strongly suggested that GtCBM104 binds to the hydrophilic regions of cellulose microfibrils, while CBM1 recognizes the hydrophobic surface. The discovery of GtCBM104 strongly suggests that the contribution of CBD to the cellulose enzymatic degradation mechanism of brown-rot fungi is much larger than previously thought. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Citation: Yuka Kojima, Naoki Sunagawa, Satomi Tagawa, Tomohiro Hatano, Moeri Aoki, Tatsuki Kurei, Yoshiki Horikawa, Masahisa Wada, Ryo Funada, Kiyohiko Igarashi, Makoto Yoshida, A cellulose-binding domain specific for native crystalline cellulose in lytic polysaccharide monooxygenase from the brown-rot fungus Gloeophyllum trabeum, Carbohydrate Polymers, 347, 122651-122651, 2025-01, https://doi.org/10.1016/j.carbpol.2024.122651 |
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言語 |
en |
| bibliographic_information |
en : Carbohydrate Polymers
巻 347,
p. 122651,
発行日 2025-01-01
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| 出版者 |
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出版者 |
Elsevier BV |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0144-8617 |
| item_10001_relation_14 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.carbpol.2024.122651 |
| 権利 |
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権利情報 |
© 2024 The Authors. |
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言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
Fulltext (7.2 MB)
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