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  1. 農学部
  1. 農学部
  2. 学術雑誌掲載論文 (農学部)
  1. 地域資源創成学部
  1. 地域資源創成学部
  2. 学術雑誌掲載論文(地域資源創成学部)

Exploration of Biomass-related Genes in the FOX-superroot Lines of Lotus corniculatus and its Impact on Agronomic Traits

http://hdl.handle.net/10458/0002001190
http://hdl.handle.net/10458/0002001190
629a880f-6ba9-49ed-803c-145a489d075c
名前 / ファイル ライセンス アクション
Final-attachment-published-LRF-813-6089603e9087340b9bdcbbb1.pdf article (773 KB)
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アイテムタイプ 学術雑誌論文 / Journal Article(1)
公開日 2025-04-22
タイトル
タイトル Exploration of Biomass-related Genes in the FOX-superroot Lines of Lotus corniculatus and its Impact on Agronomic Traits
言語 en
言語
言語 eng
キーワード
言語 en
キーワード Biomass-related genes
キーワード
言語 en
キーワード CPD
キーワード
言語 en
キーワード FOX gene-hunting system
キーワード
言語 en
キーワード Lotus corniculatus
キーワード
言語 en
キーワード SYNC1
資源タイプ
資源タイプ journal article
アクセス権
アクセス権 open access
著者 Arifin, Azeri Gautama

× Arifin, Azeri Gautama

en Arifin, Azeri Gautama

ja Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Nishi Gakuen-kibanadai, Miyazaki, 889-2192, Japan

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Shimamoto, Yasuyo

× Shimamoto, Yasuyo

en Shimamoto, Yasuyo

ja Faculty of Agriculture, University of Miyazaki, 1-1 Nishi Gakuen-kibanadai, Miyazaki, 889-2192, Japan

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Tanaka, Hidenori

× Tanaka, Hidenori

en Tanaka, Hidenori

ja Faculty of Agriculture, University of Miyazaki, 1-1 Nishi Gakuen-kibanadai, Miyazaki, 889-2192, Japan

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橋口, 正嗣

× 橋口, 正嗣

WEKO 21361
e-Rad_Researcher 50381091

ja 橋口, 正嗣
宮崎大学

ja-Kana ハシグチ, マサツグ

en Hashiguchi, Masatsugu
University of Miyazaki

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明石, 良

× 明石, 良

WEKO 1585
e-Rad_Researcher 20253809

ja 明石, 良
宮崎大学

ja-Kana アカシ, リョウ

en Akashi, Ryo
University of Miyazaki

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権藤, 崇裕

× 権藤, 崇裕

WEKO 2027
e-Rad_Researcher 10437949

ja 権藤, 崇裕
宮崎大学

ja-Kana ゴンドウ, タカヒロ

en Gondo, Takahiro
University of Miyazaki

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内容記述タイプ Abstract
内容記述 Background: Identifying biomass-related genes to meet the increasing demand for food supply is important approach for agricultural and scientific efforts. In this study, we employed the Full-length cDNA Over-eXpressing gene hunting system (FOX hunting system) to identify biomass-related genes through ectopic expression of Arabidopsis thaliana full-length cDNA in bird’s-foot trefoil (Lotus corniculatus). Methods: A total of 66 FOX superroot lines (FSLs) from Lotus corniculatus cv. Viking were transferred in vermiculite after two weeks in half-strength Murashige and Skoog (MS) medium. These FSLs were then grown in a growth chamber for four weeks, during which their plant length and root length were measured. High biomass lines were selected from this group. FSL #83 and #121 were then grown for an additional four months in a greenhouse until they reached maturity, at which point they were evaluated for their agronomic traits. Result: Out of the 66 analyzed FSLs, 15 displayed superior growth characteristics in plant length and total root length. Arabidopsis cDNA, categorized into genes related to various functions such as membrane transport, photosystems, phytohormone synthesis, amino acid synthesis and unknown genes, was introduced into these lines. Two lines were grown to maturity in the greenhouse and their agronomic traits were analyzed in detail. FSL #83 expressed brassinosteroid biosynthetic pathway gene (CPD) led to significant differences in internode length, number of stems, number of flowers and above-ground dry matter weight compared to non-transgenic Super-growing roots (SR). On the other hand, FSL #121 expressed Asparaginyl-tRNA synthetase (SYNC1) gene exhibited increases in plant length and the number of stem nodes
言語 en
内容記述
内容記述タイプ Other
内容記述 Citation:
Arifin Gautama Azeri, Shimamoto Yasuyo, Tanaka Hidenori, Hashiguchi Masatsugu, Akashi Ryo, Gondo Takahiro (2025). Exploration of Biomass-related Genes in the FOX-superroot Lines of Lotus corniculatus and its Impact on Agronomic Traits . Legume Research. 48(1): 57-63. doi: 10.18805/LRF-813.
言語 en
bibliographic_information en : Legume Research

巻 48, 号 1, p. 57-63, 発行日 2025-01-01
出版者
出版者 Agricultural Research Communication Centre
言語 en
ISSN
収録物識別子タイプ PISSN
収録物識別子 0250-5371
ISSN
収録物識別子タイプ EISSN
収録物識別子 0976-0571
item_10001_relation_14
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.18805/LRF-813
出版タイプ
出版タイプ VoR
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